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Prevalence and Antimicrobial Resistance of Campylobacter coli and Campylobacter jejuni Isolated from Pig Guts, Pig Feces, and Surface Swabs from the Cutting Tables at Slaughterhouse and Taverns in Southern Benin

Campylobacter food-borne infections are a serious public health problem. In Benin, there is a proliferation of pork consumption in several forms. This study aims to determine the prevalence and the antimicrobial resistance of Campylobacter coli and Campylobacter jejuni strains isolated from pig guts...

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Autores principales: Agbankpe, Alidehou Jerrold, Kougblenou, Sylvain D., Dougnon, Tamegnon Victorien, Oussou, Alida, Gbotche, Elodie, Koudokpon, Charles Hornel, Legba, Brice Boris, Baba-Moussa, Lamine, Bankole, Honore Sourou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9536969/
https://www.ncbi.nlm.nih.gov/pubmed/36212611
http://dx.doi.org/10.1155/2022/5120678
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author Agbankpe, Alidehou Jerrold
Kougblenou, Sylvain D.
Dougnon, Tamegnon Victorien
Oussou, Alida
Gbotche, Elodie
Koudokpon, Charles Hornel
Legba, Brice Boris
Baba-Moussa, Lamine
Bankole, Honore Sourou
author_facet Agbankpe, Alidehou Jerrold
Kougblenou, Sylvain D.
Dougnon, Tamegnon Victorien
Oussou, Alida
Gbotche, Elodie
Koudokpon, Charles Hornel
Legba, Brice Boris
Baba-Moussa, Lamine
Bankole, Honore Sourou
author_sort Agbankpe, Alidehou Jerrold
collection PubMed
description Campylobacter food-borne infections are a serious public health problem. In Benin, there is a proliferation of pork consumption in several forms. This study aims to determine the prevalence and the antimicrobial resistance of Campylobacter coli and Campylobacter jejuni strains isolated from pig guts, pig feces, and surface swabs from the cutting tables in southern Benin. For this purpose, 200 samples of pig guts, 40 samples of swabs from the cutting table surface, and 8 samples of pig feces were collected and subjected to bacteriological examination. The method used for the identification of bacteria was microbiological culture combined with molecular identification by PCR. The identified strains were then subjected to antibiotic susceptibility testing according to the methodology recommended by the EUCAST. Antibiotic profiles were compared between strains isolated from pig guts, pig feces, and cutting table surfaces on the one hand and among the different sampling sites on the other hand. The results obtained show that 47.6% of the samples analyzed were contaminated by Campylobacter spp. Molecular identification revealed 34.7% of Campylobacter coli and 9.3% of Campylobacter jejuni. The study of antimicrobial susceptibility showed resistance to ciprofloxacin, 44% to ampicillin, 23.9% to erythromycin, 11% to gentamicin, and 10.1% to amoxicillin + clavulanic acid. In total, 90.8% of the isolated Campylobacter strains were multidrug resistant. The use of antimicrobials in livestock production systems has increased considerably, which could explain, at least partially, the prevalence of Campylobacter and the resistance of strains to antibiotics. To limit the risk of Campylobacter food-borne infections, it is therefore important to include Campylobacter in the list of pathogens to be tested during sanitary quality control of meat and meat products in Benin.
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spelling pubmed-95369692022-10-07 Prevalence and Antimicrobial Resistance of Campylobacter coli and Campylobacter jejuni Isolated from Pig Guts, Pig Feces, and Surface Swabs from the Cutting Tables at Slaughterhouse and Taverns in Southern Benin Agbankpe, Alidehou Jerrold Kougblenou, Sylvain D. Dougnon, Tamegnon Victorien Oussou, Alida Gbotche, Elodie Koudokpon, Charles Hornel Legba, Brice Boris Baba-Moussa, Lamine Bankole, Honore Sourou Int J Microbiol Research Article Campylobacter food-borne infections are a serious public health problem. In Benin, there is a proliferation of pork consumption in several forms. This study aims to determine the prevalence and the antimicrobial resistance of Campylobacter coli and Campylobacter jejuni strains isolated from pig guts, pig feces, and surface swabs from the cutting tables in southern Benin. For this purpose, 200 samples of pig guts, 40 samples of swabs from the cutting table surface, and 8 samples of pig feces were collected and subjected to bacteriological examination. The method used for the identification of bacteria was microbiological culture combined with molecular identification by PCR. The identified strains were then subjected to antibiotic susceptibility testing according to the methodology recommended by the EUCAST. Antibiotic profiles were compared between strains isolated from pig guts, pig feces, and cutting table surfaces on the one hand and among the different sampling sites on the other hand. The results obtained show that 47.6% of the samples analyzed were contaminated by Campylobacter spp. Molecular identification revealed 34.7% of Campylobacter coli and 9.3% of Campylobacter jejuni. The study of antimicrobial susceptibility showed resistance to ciprofloxacin, 44% to ampicillin, 23.9% to erythromycin, 11% to gentamicin, and 10.1% to amoxicillin + clavulanic acid. In total, 90.8% of the isolated Campylobacter strains were multidrug resistant. The use of antimicrobials in livestock production systems has increased considerably, which could explain, at least partially, the prevalence of Campylobacter and the resistance of strains to antibiotics. To limit the risk of Campylobacter food-borne infections, it is therefore important to include Campylobacter in the list of pathogens to be tested during sanitary quality control of meat and meat products in Benin. Hindawi 2022-09-29 /pmc/articles/PMC9536969/ /pubmed/36212611 http://dx.doi.org/10.1155/2022/5120678 Text en Copyright © 2022 Alidehou Jerrold Agbankpe et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Agbankpe, Alidehou Jerrold
Kougblenou, Sylvain D.
Dougnon, Tamegnon Victorien
Oussou, Alida
Gbotche, Elodie
Koudokpon, Charles Hornel
Legba, Brice Boris
Baba-Moussa, Lamine
Bankole, Honore Sourou
Prevalence and Antimicrobial Resistance of Campylobacter coli and Campylobacter jejuni Isolated from Pig Guts, Pig Feces, and Surface Swabs from the Cutting Tables at Slaughterhouse and Taverns in Southern Benin
title Prevalence and Antimicrobial Resistance of Campylobacter coli and Campylobacter jejuni Isolated from Pig Guts, Pig Feces, and Surface Swabs from the Cutting Tables at Slaughterhouse and Taverns in Southern Benin
title_full Prevalence and Antimicrobial Resistance of Campylobacter coli and Campylobacter jejuni Isolated from Pig Guts, Pig Feces, and Surface Swabs from the Cutting Tables at Slaughterhouse and Taverns in Southern Benin
title_fullStr Prevalence and Antimicrobial Resistance of Campylobacter coli and Campylobacter jejuni Isolated from Pig Guts, Pig Feces, and Surface Swabs from the Cutting Tables at Slaughterhouse and Taverns in Southern Benin
title_full_unstemmed Prevalence and Antimicrobial Resistance of Campylobacter coli and Campylobacter jejuni Isolated from Pig Guts, Pig Feces, and Surface Swabs from the Cutting Tables at Slaughterhouse and Taverns in Southern Benin
title_short Prevalence and Antimicrobial Resistance of Campylobacter coli and Campylobacter jejuni Isolated from Pig Guts, Pig Feces, and Surface Swabs from the Cutting Tables at Slaughterhouse and Taverns in Southern Benin
title_sort prevalence and antimicrobial resistance of campylobacter coli and campylobacter jejuni isolated from pig guts, pig feces, and surface swabs from the cutting tables at slaughterhouse and taverns in southern benin
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9536969/
https://www.ncbi.nlm.nih.gov/pubmed/36212611
http://dx.doi.org/10.1155/2022/5120678
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