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A universal SARS‐CoV DNA vaccine inducing highly cross‐reactive neutralizing antibodies and T cells

New variants in the SARS‐CoV‐2 pandemic are more contagious (Alpha/Delta), evade neutralizing antibodies (Beta), or both (Omicron). This poses a challenge in vaccine development according to WHO. We designed a more universal SARS‐CoV‐2 DNA vaccine containing receptor‐binding domain loops from the hu...

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Autores principales: Appelberg, Sofia, Ahlén, Gustaf, Yan, Jingyi, Nikouyan, Negin, Weber, Sofie, Larsson, Olivia, Höglund, Urban, Aleman, Soo, Weber, Friedemann, Perlhamre, Emma, Apro, Johanna, Gidlund, Eva‐Karin, Tuvesson, Ola, Salati, Simona, Cadossi, Matteo, Tegel, Hanna, Hober, Sophia, Frelin, Lars, Mirazimi, Ali, Sällberg, Matti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9538582/
https://www.ncbi.nlm.nih.gov/pubmed/35986481
http://dx.doi.org/10.15252/emmm.202215821
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author Appelberg, Sofia
Ahlén, Gustaf
Yan, Jingyi
Nikouyan, Negin
Weber, Sofie
Larsson, Olivia
Höglund, Urban
Aleman, Soo
Weber, Friedemann
Perlhamre, Emma
Apro, Johanna
Gidlund, Eva‐Karin
Tuvesson, Ola
Salati, Simona
Cadossi, Matteo
Tegel, Hanna
Hober, Sophia
Frelin, Lars
Mirazimi, Ali
Sällberg, Matti
author_facet Appelberg, Sofia
Ahlén, Gustaf
Yan, Jingyi
Nikouyan, Negin
Weber, Sofie
Larsson, Olivia
Höglund, Urban
Aleman, Soo
Weber, Friedemann
Perlhamre, Emma
Apro, Johanna
Gidlund, Eva‐Karin
Tuvesson, Ola
Salati, Simona
Cadossi, Matteo
Tegel, Hanna
Hober, Sophia
Frelin, Lars
Mirazimi, Ali
Sällberg, Matti
author_sort Appelberg, Sofia
collection PubMed
description New variants in the SARS‐CoV‐2 pandemic are more contagious (Alpha/Delta), evade neutralizing antibodies (Beta), or both (Omicron). This poses a challenge in vaccine development according to WHO. We designed a more universal SARS‐CoV‐2 DNA vaccine containing receptor‐binding domain loops from the huCoV‐19/WH01, the Alpha, and the Beta variants, combined with the membrane and nucleoproteins. The vaccine induced spike antibodies crossreactive between huCoV‐19/WH01, Beta, and Delta spike proteins that neutralized huCoV‐19/WH01, Beta, Delta, and Omicron virus in vitro. The vaccine primed nucleoprotein‐specific T cells, unlike spike‐specific T cells, recognized Bat‐CoV sequences. The vaccine protected mice carrying the human ACE2 receptor against lethal infection with the SARS‐CoV‐2 Beta variant. Interestingly, priming of cross‐reactive nucleoprotein‐specific T cells alone was 60% protective, verifying observations from humans that T cells protect against lethal disease. This SARS‐CoV vaccine induces a uniquely broad and functional immunity that adds to currently used vaccines.
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spelling pubmed-95385822022-10-11 A universal SARS‐CoV DNA vaccine inducing highly cross‐reactive neutralizing antibodies and T cells Appelberg, Sofia Ahlén, Gustaf Yan, Jingyi Nikouyan, Negin Weber, Sofie Larsson, Olivia Höglund, Urban Aleman, Soo Weber, Friedemann Perlhamre, Emma Apro, Johanna Gidlund, Eva‐Karin Tuvesson, Ola Salati, Simona Cadossi, Matteo Tegel, Hanna Hober, Sophia Frelin, Lars Mirazimi, Ali Sällberg, Matti EMBO Mol Med Report New variants in the SARS‐CoV‐2 pandemic are more contagious (Alpha/Delta), evade neutralizing antibodies (Beta), or both (Omicron). This poses a challenge in vaccine development according to WHO. We designed a more universal SARS‐CoV‐2 DNA vaccine containing receptor‐binding domain loops from the huCoV‐19/WH01, the Alpha, and the Beta variants, combined with the membrane and nucleoproteins. The vaccine induced spike antibodies crossreactive between huCoV‐19/WH01, Beta, and Delta spike proteins that neutralized huCoV‐19/WH01, Beta, Delta, and Omicron virus in vitro. The vaccine primed nucleoprotein‐specific T cells, unlike spike‐specific T cells, recognized Bat‐CoV sequences. The vaccine protected mice carrying the human ACE2 receptor against lethal infection with the SARS‐CoV‐2 Beta variant. Interestingly, priming of cross‐reactive nucleoprotein‐specific T cells alone was 60% protective, verifying observations from humans that T cells protect against lethal disease. This SARS‐CoV vaccine induces a uniquely broad and functional immunity that adds to currently used vaccines. John Wiley and Sons Inc. 2022-09-02 /pmc/articles/PMC9538582/ /pubmed/35986481 http://dx.doi.org/10.15252/emmm.202215821 Text en © 2022 The Authors. Published under the terms of the CC BY 4.0 license. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Report
Appelberg, Sofia
Ahlén, Gustaf
Yan, Jingyi
Nikouyan, Negin
Weber, Sofie
Larsson, Olivia
Höglund, Urban
Aleman, Soo
Weber, Friedemann
Perlhamre, Emma
Apro, Johanna
Gidlund, Eva‐Karin
Tuvesson, Ola
Salati, Simona
Cadossi, Matteo
Tegel, Hanna
Hober, Sophia
Frelin, Lars
Mirazimi, Ali
Sällberg, Matti
A universal SARS‐CoV DNA vaccine inducing highly cross‐reactive neutralizing antibodies and T cells
title A universal SARS‐CoV DNA vaccine inducing highly cross‐reactive neutralizing antibodies and T cells
title_full A universal SARS‐CoV DNA vaccine inducing highly cross‐reactive neutralizing antibodies and T cells
title_fullStr A universal SARS‐CoV DNA vaccine inducing highly cross‐reactive neutralizing antibodies and T cells
title_full_unstemmed A universal SARS‐CoV DNA vaccine inducing highly cross‐reactive neutralizing antibodies and T cells
title_short A universal SARS‐CoV DNA vaccine inducing highly cross‐reactive neutralizing antibodies and T cells
title_sort universal sars‐cov dna vaccine inducing highly cross‐reactive neutralizing antibodies and t cells
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9538582/
https://www.ncbi.nlm.nih.gov/pubmed/35986481
http://dx.doi.org/10.15252/emmm.202215821
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