Generation of Photocaged Nanobodies for Intracellular Applications in an Animal Using Genetic Code Expansion and Computationally Guided Protein Engineering

Nanobodies are becoming increasingly popular as tools for manipulating and visualising proteins in vivo. The ability to control nanobody/antigen interactions using light could provide precise spatiotemporal control over protein function. We develop a general approach to engineer photo‐activatable na...

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Detalles Bibliográficos
Autores principales: O'Shea, Jack M., Goutou, Angeliki, Brydon, Jack, Sethna, Cyrus R., Wood, Christopher W., Greiss, Sebastian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9542635/
https://www.ncbi.nlm.nih.gov/pubmed/35731601
http://dx.doi.org/10.1002/cbic.202200321

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9542635/
https://www.ncbi.nlm.nih.gov/pubmed/35731601
http://dx.doi.org/10.1002/cbic.202200321

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