Optimized quantitative PCR analysis of random DNA aptamer libraries

The quantitative polymerase chain reaction (qPCR) with detection of duplex DNA yield by intercalator fluorescence is a common and essential technique in nucleic acid analysis. We encountered unexpected results when applying standard qPCR methods to the quantitation of random DNA libraries flanked by...

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Detalles Bibliográficos
Autores principales: Pearson, Keenan, Doherty, Caroline, Zhang, Drason, Becker, Nicole A., Maher, L. James
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9542921/
https://www.ncbi.nlm.nih.gov/pubmed/35561815
http://dx.doi.org/10.1016/j.ab.2022.114712

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9542921/
https://www.ncbi.nlm.nih.gov/pubmed/35561815
http://dx.doi.org/10.1016/j.ab.2022.114712

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