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Optimized quantitative PCR analysis of random DNA aptamer libraries

The quantitative polymerase chain reaction (qPCR) with detection of duplex DNA yield by intercalator fluorescence is a common and essential technique in nucleic acid analysis. We encountered unexpected results when applying standard qPCR methods to the quantitation of random DNA libraries flanked by...

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Detalles Bibliográficos
Autores principales:	Pearson, Keenan, Doherty, Caroline, Zhang, Drason, Becker, Nicole A., Maher, L. James
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9542921/ https://www.ncbi.nlm.nih.gov/pubmed/35561815 http://dx.doi.org/10.1016/j.ab.2022.114712

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