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Generating and testing the efficacy of transgenic Cas9 in Tribolium castaneum

CRISPR/Cas9 genome editing has now expanded to many insect species, including Tribolium castaneum. However, compared to Drosophila melanogaster, the CRISPR toolkit of T. castaneum is limited. A particularly apparent gap is the lack of Cas9 transgenic animals, which generally offer higher editing eff...

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Detalles Bibliográficos
Autores principales: Rylee, Johnathan C., Nin‐Velez, Alexandra, Mahato, Simpla, Helms, Kennedy J., Wade, Michael J., Zentner, Gabriel E., Zelhof, Andrew C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Ltd. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9544626/
https://www.ncbi.nlm.nih.gov/pubmed/35429082
http://dx.doi.org/10.1111/imb.12778