A split green fluorescent protein system to enhance spatial and temporal sensitivity of translating ribosome affinity purification

Translating ribosome affinity purification (TRAP) utilizes transgenic plants expressing a ribosomal protein fused to a tag for affinity co‐purification of ribosomes and the mRNAs that they are translating. This population of actively translated mRNAs (translatome) can be interrogated by quantitative...

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Detalles Bibliográficos
Autores principales: Dinkeloo, Kasia, Pelly, Zoe, McDowell, John M., Pilot, Guillaume
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Technical Advance
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9544980/
https://www.ncbi.nlm.nih.gov/pubmed/35436375
http://dx.doi.org/10.1111/tpj.15779

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9544980/
https://www.ncbi.nlm.nih.gov/pubmed/35436375
http://dx.doi.org/10.1111/tpj.15779

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