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A split green fluorescent protein system to enhance spatial and temporal sensitivity of translating ribosome affinity purification

Translating ribosome affinity purification (TRAP) utilizes transgenic plants expressing a ribosomal protein fused to a tag for affinity co‐purification of ribosomes and the mRNAs that they are translating. This population of actively translated mRNAs (translatome) can be interrogated by quantitative...

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Detalles Bibliográficos
Autores principales:	Dinkeloo, Kasia, Pelly, Zoe, McDowell, John M., Pilot, Guillaume
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2022
Materias:	Technical Advance
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9544980/ https://www.ncbi.nlm.nih.gov/pubmed/35436375 http://dx.doi.org/10.1111/tpj.15779

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