A native promoter–gene fusion created by CRISPR/Cas9‐mediated genomic deletion offers a transgene‐free method to drive oil accumulation in leaves

Achieving gain‐of‐function phenotypes without inserting foreign DNA is an important challenge for plant biotechnologists. Here, we show that a gene can be brought under the control of a promoter from an upstream gene by deleting the intervening genomic sequence using dual‐guide CRISPR/Cas9. We fuse...

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Detalles Bibliográficos
Autores principales: Bhunia, Rupam Kumar, Menard, Guillaume N., Eastmond, Peter J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Research Letters
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9545981/
https://www.ncbi.nlm.nih.gov/pubmed/35490366
http://dx.doi.org/10.1002/1873-3468.14365

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9545981/
https://www.ncbi.nlm.nih.gov/pubmed/35490366
http://dx.doi.org/10.1002/1873-3468.14365

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