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Scalable single-cell RNA sequencing from full transcripts with Smart-seq3xpress

Current single-cell RNA sequencing (scRNA-seq) methods with high cellular throughputs sacrifice full-transcript coverage and often sensitivity. Here we describe Smart-seq3xpress, which miniaturizes and streamlines the Smart-seq3 protocol to substantially reduce reagent use and increase cellular thro...

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Detalles Bibliográficos
Autores principales: Hagemann-Jensen, Michael, Ziegenhain, Christoph, Sandberg, Rickard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9546772/
https://www.ncbi.nlm.nih.gov/pubmed/35637418
http://dx.doi.org/10.1038/s41587-022-01311-4