Image-guided radiotherapy in an orthotopic mouse model of rectal cancer

Radiobiology research in rectal cancer has been limited to cell lines, patient-derived organoids (PDOs), or xenografts. Here, we describe a protocol which recapitulates more efficiently the complex contributions of the tumor microenvironment. This approach establishes a preclinical mouse model of re...

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Autores principales: Nicolas, Adele M., Pesic, Marina, Rödel, Franz, Fokas, Emmanouil, Greten, Florian R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9547293/
https://www.ncbi.nlm.nih.gov/pubmed/36206161
http://dx.doi.org/10.1016/j.xpro.2022.101749
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author Nicolas, Adele M.
Pesic, Marina
Rödel, Franz
Fokas, Emmanouil
Greten, Florian R.
author_facet Nicolas, Adele M.
Pesic, Marina
Rödel, Franz
Fokas, Emmanouil
Greten, Florian R.
author_sort Nicolas, Adele M.
collection PubMed
description Radiobiology research in rectal cancer has been limited to cell lines, patient-derived organoids (PDOs), or xenografts. Here, we describe a protocol which recapitulates more efficiently the complex contributions of the tumor microenvironment. This approach establishes a preclinical mouse model of rectal cancer by intrarectal transplantation of genetically modified organoids into immunocompetent mice followed by precise image-guided radiotherapy (IGRT) of organoid-induced tumors. This model represents a useful platform to study the cellular and molecular determinants of therapy resistance in rectal cancer. For complete details on the use and execution of this protocol, please refer to Nicolas et al. (2022).
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spelling pubmed-95472932022-10-09 Image-guided radiotherapy in an orthotopic mouse model of rectal cancer Nicolas, Adele M. Pesic, Marina Rödel, Franz Fokas, Emmanouil Greten, Florian R. STAR Protoc Protocol Radiobiology research in rectal cancer has been limited to cell lines, patient-derived organoids (PDOs), or xenografts. Here, we describe a protocol which recapitulates more efficiently the complex contributions of the tumor microenvironment. This approach establishes a preclinical mouse model of rectal cancer by intrarectal transplantation of genetically modified organoids into immunocompetent mice followed by precise image-guided radiotherapy (IGRT) of organoid-induced tumors. This model represents a useful platform to study the cellular and molecular determinants of therapy resistance in rectal cancer. For complete details on the use and execution of this protocol, please refer to Nicolas et al. (2022). Elsevier 2022-10-05 /pmc/articles/PMC9547293/ /pubmed/36206161 http://dx.doi.org/10.1016/j.xpro.2022.101749 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Nicolas, Adele M.
Pesic, Marina
Rödel, Franz
Fokas, Emmanouil
Greten, Florian R.
Image-guided radiotherapy in an orthotopic mouse model of rectal cancer
title Image-guided radiotherapy in an orthotopic mouse model of rectal cancer
title_full Image-guided radiotherapy in an orthotopic mouse model of rectal cancer
title_fullStr Image-guided radiotherapy in an orthotopic mouse model of rectal cancer
title_full_unstemmed Image-guided radiotherapy in an orthotopic mouse model of rectal cancer
title_short Image-guided radiotherapy in an orthotopic mouse model of rectal cancer
title_sort image-guided radiotherapy in an orthotopic mouse model of rectal cancer
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9547293/
https://www.ncbi.nlm.nih.gov/pubmed/36206161
http://dx.doi.org/10.1016/j.xpro.2022.101749
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