Toward efficient and high-fidelity metagenomic data from sub-nanogram DNA: evaluation of library preparation and decontamination methods

BACKGROUND: Shotgun metagenomic sequencing has greatly expanded the understanding of microbial communities in various biological niches. However, it is still challenging to efficiently convert sub-nanogram DNA to high-quality metagenomic libraries and obtain high-fidelity data, hindering the explora...

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Detalles Bibliográficos
Autores principales: Wang, Chun, Zhang, Li, Jiang, Xuan, Ma, Wentai, Geng, Hui, Wang, Xue, Li, Mingkun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9548135/
https://www.ncbi.nlm.nih.gov/pubmed/36209213
http://dx.doi.org/10.1186/s12915-022-01418-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9548135/
https://www.ncbi.nlm.nih.gov/pubmed/36209213
http://dx.doi.org/10.1186/s12915-022-01418-9

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