Enhanced mitochondrial DNA editing in mice using nuclear-exported TALE-linked deaminases and nucleases

We present two methods for enhancing the efficiency of mitochondrial DNA (mtDNA) editing in mice with DddA-derived cytosine base editors (DdCBEs). First, we fused DdCBEs to a nuclear export signal (DdCBE-NES) to avoid off-target C-to-T conversions in the nuclear genome and improve editing efficiency...

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Detalles Bibliográficos
Autores principales: Lee, Seonghyun, Lee, Hyunji, Baek, Gayoung, Namgung, Eunji, Park, Joo Min, Kim, Sanghun, Hong, Seongho, Kim, Jin-Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9554978/
https://www.ncbi.nlm.nih.gov/pubmed/36224582
http://dx.doi.org/10.1186/s13059-022-02782-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9554978/
https://www.ncbi.nlm.nih.gov/pubmed/36224582
http://dx.doi.org/10.1186/s13059-022-02782-z

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