DAR-PCR: a new tool for efficient retrieval of unknown flanking genomic DNA

Various PCR-based genome-walking methods have been developed to acquire unknown flanking DNA sequences. However, the specificity and efficacy levels, and the operational processes, of the available methods are unsatisfactory. This work proposes a novel walking approach, termed differential annealing...

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Detalles Bibliográficos
Autores principales: Sun, Tianyi, Jia, Mengya, Wang, Lingqin, Li, Zhaoqin, Lin, Zhiyu, Wei, Cheng, Pei, Jinfeng, Li, Haixing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9556680/
https://www.ncbi.nlm.nih.gov/pubmed/36224448
http://dx.doi.org/10.1186/s13568-022-01471-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9556680/
https://www.ncbi.nlm.nih.gov/pubmed/36224448
http://dx.doi.org/10.1186/s13568-022-01471-1

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