Cargando…
DAR-PCR: a new tool for efficient retrieval of unknown flanking genomic DNA
Various PCR-based genome-walking methods have been developed to acquire unknown flanking DNA sequences. However, the specificity and efficacy levels, and the operational processes, of the available methods are unsatisfactory. This work proposes a novel walking approach, termed differential annealing...
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer Berlin Heidelberg
2022
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9556680/ https://www.ncbi.nlm.nih.gov/pubmed/36224448 http://dx.doi.org/10.1186/s13568-022-01471-1 |