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Altered Behavioral Responses Show GABA Sensitivity in Muscleblind-Like 2-Deficient Mice: Implications for CNS Symptoms in Myotonic Dystrophy

Considerable evidence from mouse models and human postmortem brain suggests loss of Muscleblind-like protein 2 (MBNL2) function in brain is a major driver of CNS symptoms in Myotonic dystrophy type 1 (DM1). Increased hypersomnia, fatigue, and surgical complications associated with general anesthesia...

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Autores principales: Edokpolor, Kamyra S., Banerjee, Anwesha, McEachin, Zachary T., Gu, Jingsheng, Kosti, Adam, Arboleda, Juan D., García, Paul S., Wang, Eric T., Bassell, Gary J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society for Neuroscience 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9557336/
https://www.ncbi.nlm.nih.gov/pubmed/36150891
http://dx.doi.org/10.1523/ENEURO.0218-22.2022
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author Edokpolor, Kamyra S.
Banerjee, Anwesha
McEachin, Zachary T.
Gu, Jingsheng
Kosti, Adam
Arboleda, Juan D.
García, Paul S.
Wang, Eric T.
Bassell, Gary J.
author_facet Edokpolor, Kamyra S.
Banerjee, Anwesha
McEachin, Zachary T.
Gu, Jingsheng
Kosti, Adam
Arboleda, Juan D.
García, Paul S.
Wang, Eric T.
Bassell, Gary J.
author_sort Edokpolor, Kamyra S.
collection PubMed
description Considerable evidence from mouse models and human postmortem brain suggests loss of Muscleblind-like protein 2 (MBNL2) function in brain is a major driver of CNS symptoms in Myotonic dystrophy type 1 (DM1). Increased hypersomnia, fatigue, and surgical complications associated with general anesthesia suggest possible sensitivity to GABAergic inhibition in DM1. To test the hypothesis that MBNL2 depletion leads to behavioral sensitivity to GABA(A) receptor (GABA(A)-R) modulation, Mbnl2 knock-out (KO) and wild-type (WT) littermates were treated with the anesthetic sevoflurane, the benzodiazepine diazepam, the imidazopyridine zolpidem, and the benzodiazepine rescue agent, flumazenil (Ro 15-1788), and assessed for various behavioral metrics. Mbnl2 KO mice exhibited delayed recovery following sevoflurane, delayed emergence and recovery from zolpidem, and enhanced sleep time at baseline that was modulated by flumazenil. A significantly higher proportion of Mbnl2 KO mice also loss their righting reflex [loss of righting reflex (LORR)] from a standard diazepam dose. We further examined whether MBNL2 depletion affects total GABA(A)-R mRNA subunit levels and validated RNA-sequencing data of mis-spliced Gabrg2, whose isoform ratios are known to regulate GABA sensitivity and associated behaviors. While no other GABA(A)-R subunit mRNA levels tested were altered in Mbnl2 KO mouse prefrontal cortex, Gabrg2S/L mRNA ratio levels were significantly altered. Taken together, our findings indicate that loss of MBNL2 function affects GABAergic function in a mouse model of myotonic dystrophy (DM1).
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spelling pubmed-95573362022-10-13 Altered Behavioral Responses Show GABA Sensitivity in Muscleblind-Like 2-Deficient Mice: Implications for CNS Symptoms in Myotonic Dystrophy Edokpolor, Kamyra S. Banerjee, Anwesha McEachin, Zachary T. Gu, Jingsheng Kosti, Adam Arboleda, Juan D. García, Paul S. Wang, Eric T. Bassell, Gary J. eNeuro Research Article: New Research Considerable evidence from mouse models and human postmortem brain suggests loss of Muscleblind-like protein 2 (MBNL2) function in brain is a major driver of CNS symptoms in Myotonic dystrophy type 1 (DM1). Increased hypersomnia, fatigue, and surgical complications associated with general anesthesia suggest possible sensitivity to GABAergic inhibition in DM1. To test the hypothesis that MBNL2 depletion leads to behavioral sensitivity to GABA(A) receptor (GABA(A)-R) modulation, Mbnl2 knock-out (KO) and wild-type (WT) littermates were treated with the anesthetic sevoflurane, the benzodiazepine diazepam, the imidazopyridine zolpidem, and the benzodiazepine rescue agent, flumazenil (Ro 15-1788), and assessed for various behavioral metrics. Mbnl2 KO mice exhibited delayed recovery following sevoflurane, delayed emergence and recovery from zolpidem, and enhanced sleep time at baseline that was modulated by flumazenil. A significantly higher proportion of Mbnl2 KO mice also loss their righting reflex [loss of righting reflex (LORR)] from a standard diazepam dose. We further examined whether MBNL2 depletion affects total GABA(A)-R mRNA subunit levels and validated RNA-sequencing data of mis-spliced Gabrg2, whose isoform ratios are known to regulate GABA sensitivity and associated behaviors. While no other GABA(A)-R subunit mRNA levels tested were altered in Mbnl2 KO mouse prefrontal cortex, Gabrg2S/L mRNA ratio levels were significantly altered. Taken together, our findings indicate that loss of MBNL2 function affects GABAergic function in a mouse model of myotonic dystrophy (DM1). Society for Neuroscience 2022-10-07 /pmc/articles/PMC9557336/ /pubmed/36150891 http://dx.doi.org/10.1523/ENEURO.0218-22.2022 Text en Copyright © 2022 Edokpolor et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article: New Research
Edokpolor, Kamyra S.
Banerjee, Anwesha
McEachin, Zachary T.
Gu, Jingsheng
Kosti, Adam
Arboleda, Juan D.
García, Paul S.
Wang, Eric T.
Bassell, Gary J.
Altered Behavioral Responses Show GABA Sensitivity in Muscleblind-Like 2-Deficient Mice: Implications for CNS Symptoms in Myotonic Dystrophy
title Altered Behavioral Responses Show GABA Sensitivity in Muscleblind-Like 2-Deficient Mice: Implications for CNS Symptoms in Myotonic Dystrophy
title_full Altered Behavioral Responses Show GABA Sensitivity in Muscleblind-Like 2-Deficient Mice: Implications for CNS Symptoms in Myotonic Dystrophy
title_fullStr Altered Behavioral Responses Show GABA Sensitivity in Muscleblind-Like 2-Deficient Mice: Implications for CNS Symptoms in Myotonic Dystrophy
title_full_unstemmed Altered Behavioral Responses Show GABA Sensitivity in Muscleblind-Like 2-Deficient Mice: Implications for CNS Symptoms in Myotonic Dystrophy
title_short Altered Behavioral Responses Show GABA Sensitivity in Muscleblind-Like 2-Deficient Mice: Implications for CNS Symptoms in Myotonic Dystrophy
title_sort altered behavioral responses show gaba sensitivity in muscleblind-like 2-deficient mice: implications for cns symptoms in myotonic dystrophy
topic Research Article: New Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9557336/
https://www.ncbi.nlm.nih.gov/pubmed/36150891
http://dx.doi.org/10.1523/ENEURO.0218-22.2022
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