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Optimized protocol for obtaining and characterizing primary neuron-enriched cultures from embryonic chicken brains
We present here an optimized protocol to obtain primary neuron-enriched cultures from embryonic chicken brains with no need for an animal facility. The protocol details the steps to isolate a neuron-enriched cell fraction from chicken embryos, followed by characterization of the chicken neurons with...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9558106/ https://www.ncbi.nlm.nih.gov/pubmed/36209426 http://dx.doi.org/10.1016/j.xpro.2022.101753 |