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Optimized protocol for obtaining and characterizing primary neuron-enriched cultures from embryonic chicken brains

We present here an optimized protocol to obtain primary neuron-enriched cultures from embryonic chicken brains with no need for an animal facility. The protocol details the steps to isolate a neuron-enriched cell fraction from chicken embryos, followed by characterization of the chicken neurons with...

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Detalles Bibliográficos
Autores principales: Marques, Cátia F., Pinheiro, Pedro F., Justino, Gonçalo C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9558106/
https://www.ncbi.nlm.nih.gov/pubmed/36209426
http://dx.doi.org/10.1016/j.xpro.2022.101753