Optimized protocol for obtaining and characterizing primary neuron-enriched cultures from embryonic chicken brains

We present here an optimized protocol to obtain primary neuron-enriched cultures from embryonic chicken brains with no need for an animal facility. The protocol details the steps to isolate a neuron-enriched cell fraction from chicken embryos, followed by characterization of the chicken neurons with...

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Detalles Bibliográficos
Autores principales: Marques, Cátia F., Pinheiro, Pedro F., Justino, Gonçalo C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9558106/
https://www.ncbi.nlm.nih.gov/pubmed/36209426
http://dx.doi.org/10.1016/j.xpro.2022.101753
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author Marques, Cátia F.
Pinheiro, Pedro F.
Justino, Gonçalo C.
author_facet Marques, Cátia F.
Pinheiro, Pedro F.
Justino, Gonçalo C.
author_sort Marques, Cátia F.
collection PubMed
description We present here an optimized protocol to obtain primary neuron-enriched cultures from embryonic chicken brains with no need for an animal facility. The protocol details the steps to isolate a neuron-enriched cell fraction from chicken embryos, followed by characterization of the chicken neurons with mass spectrometry proteomics and cell staining. Because of the high homology between chicken and human amyloid precursor protein processing machinery, these chicken neurons can be used as an alternative to rodent models for studying Alzheimer disease.
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spelling pubmed-95581062022-10-14 Optimized protocol for obtaining and characterizing primary neuron-enriched cultures from embryonic chicken brains Marques, Cátia F. Pinheiro, Pedro F. Justino, Gonçalo C. STAR Protoc Protocol We present here an optimized protocol to obtain primary neuron-enriched cultures from embryonic chicken brains with no need for an animal facility. The protocol details the steps to isolate a neuron-enriched cell fraction from chicken embryos, followed by characterization of the chicken neurons with mass spectrometry proteomics and cell staining. Because of the high homology between chicken and human amyloid precursor protein processing machinery, these chicken neurons can be used as an alternative to rodent models for studying Alzheimer disease. Elsevier 2022-10-07 /pmc/articles/PMC9558106/ /pubmed/36209426 http://dx.doi.org/10.1016/j.xpro.2022.101753 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Marques, Cátia F.
Pinheiro, Pedro F.
Justino, Gonçalo C.
Optimized protocol for obtaining and characterizing primary neuron-enriched cultures from embryonic chicken brains
title Optimized protocol for obtaining and characterizing primary neuron-enriched cultures from embryonic chicken brains
title_full Optimized protocol for obtaining and characterizing primary neuron-enriched cultures from embryonic chicken brains
title_fullStr Optimized protocol for obtaining and characterizing primary neuron-enriched cultures from embryonic chicken brains
title_full_unstemmed Optimized protocol for obtaining and characterizing primary neuron-enriched cultures from embryonic chicken brains
title_short Optimized protocol for obtaining and characterizing primary neuron-enriched cultures from embryonic chicken brains
title_sort optimized protocol for obtaining and characterizing primary neuron-enriched cultures from embryonic chicken brains
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9558106/
https://www.ncbi.nlm.nih.gov/pubmed/36209426
http://dx.doi.org/10.1016/j.xpro.2022.101753
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