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Amount of Cas9 protein introduced into mouse embryos via electroporation affects the genome-editing rate

Genetically engineered animals can be produced quickly using genome editing technology. A new electroporation technique, technique for animal knockout system by electroporation (TAKE), aids in the production of genome-edited animals by introducing nucleases into intact embryos using electroporation...

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Detalles Bibliográficos
Autores principales: WAKE, Yui, VAKULSKAS, Christopher A., GLENN, Steve E., KANEKO, Takehito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9558813/
https://www.ncbi.nlm.nih.gov/pubmed/35831117
http://dx.doi.org/10.1262/jrd.2022-067