Cargando…
Amount of Cas9 protein introduced into mouse embryos via electroporation affects the genome-editing rate
Genetically engineered animals can be produced quickly using genome editing technology. A new electroporation technique, technique for animal knockout system by electroporation (TAKE), aids in the production of genome-edited animals by introducing nucleases into intact embryos using electroporation...
Autores principales: | WAKE, Yui, VAKULSKAS, Christopher A., GLENN, Steve E., KANEKO, Takehito |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Society for Reproduction and Development
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9558813/ https://www.ncbi.nlm.nih.gov/pubmed/35831117 http://dx.doi.org/10.1262/jrd.2022-067 |
Ejemplares similares
-
Production of genome-edited mice by visualization of nucleases introduced into the embryos using electroporation
por: WAKE, Yui, et al.
Publicado: (2020) -
Simple Genome Editing of Rodent Intact Embryos by Electroporation
por: Kaneko, Takehito, et al.
Publicado: (2015) -
Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages
por: Le, Quynh Anh, et al.
Publicado: (2021) -
TIE: A Method to Electroporate Long DNA Templates into Preimplantation Embryos for CRISPR-Cas9 Gene Editing
por: Bagheri, Hooman, et al.
Publicado: (2018) -
Simple knockout by electroporation of engineered endonucleases into intact rat embryos
por: Kaneko, Takehito, et al.
Publicado: (2014)