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Tetra-Primer Amplification-Refractory Mutation System (ARMS)—PCR for Genotyping Mouse Leptin Gene Mutation

SIMPLE SUMMARY: We developed a method to detect a single-point mutation in an obese gene in a mouse research model. The method is quick, easy to conduct, and suitable for obesity and diabetes-related research, especially in settings with constrained resources to identify and differentiate wild-type...

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Detalles Bibliográficos
Autores principales: Chen, Jiangang, Xu, Xinyun, Dalhaimer, Paul, Zhao, Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9558987/
https://www.ncbi.nlm.nih.gov/pubmed/36230421
http://dx.doi.org/10.3390/ani12192680