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Exercise regulates shelterin genes and microRNAs implicated in ageing in Thoroughbred horses
Ageing causes a gradual deterioration of bodily functions and telomere degradation. Excessive telomere shortening leads to cellular senescence and decreases tissue vitality. Six proteins, called shelterin, protect telomere integrity and control telomere length through telomerase-dependent mechanisms...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9560944/ https://www.ncbi.nlm.nih.gov/pubmed/36085194 http://dx.doi.org/10.1007/s00424-022-02745-0 |
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author | Mandal, Shama Denham, Michele M. Spencer, Sarah J. Denham, Joshua |
author_facet | Mandal, Shama Denham, Michele M. Spencer, Sarah J. Denham, Joshua |
author_sort | Mandal, Shama |
collection | PubMed |
description | Ageing causes a gradual deterioration of bodily functions and telomere degradation. Excessive telomere shortening leads to cellular senescence and decreases tissue vitality. Six proteins, called shelterin, protect telomere integrity and control telomere length through telomerase-dependent mechanisms. Exercise training appears to maintain telomeres in certain somatic cells, although the underlying molecular mechanisms are incompletely understood. Here, we examined the influence of a single bout of vigorous exercise training on leukocyte telomerase reverse transcriptase (TERT) and shelterin gene expression, and the abundance of three microRNAs (miRNAs) implicated in biological ageing (miRNA-143, -223 and -486-5p) in an elite athlete and large animal model, Thoroughbred horses. Gene and miRNA expression were analysed using primer-based and TaqMan Assay qPCR. Leukocyte TRF1, TRF2 and POT1 expression were all significantly increased whilst miR-223 and miR-486-5p were decreased immediately after vigorous exercise (all p < 0.05), and tended to return to baseline levels 24 h after training. Relative to the young horses (~ 3.9 years old), middle-aged horses (~ 14.8 years old) exhibited reduced leukocyte TERT gene expression, and increased POT1 and miR-223 abundance (all p < 0.05). These data demonstrate that genes transcribing key components of the shelterin-telomere complex are influenced by ageing and dynamically regulated by a single bout of vigorous exercise in a large, athletic mammal — Thoroughbred horses. Our findings also implicate TERT and shelterin gene transcripts as potential targets of miR-223 and miR-486-5p, which are modulated by exercise and may have a role in the telomere maintenance and genomic stability associated with long-term aerobic training. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00424-022-02745-0. |
format | Online Article Text |
id | pubmed-9560944 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-95609442022-10-15 Exercise regulates shelterin genes and microRNAs implicated in ageing in Thoroughbred horses Mandal, Shama Denham, Michele M. Spencer, Sarah J. Denham, Joshua Pflugers Arch Molecular and Genomic Physiology Ageing causes a gradual deterioration of bodily functions and telomere degradation. Excessive telomere shortening leads to cellular senescence and decreases tissue vitality. Six proteins, called shelterin, protect telomere integrity and control telomere length through telomerase-dependent mechanisms. Exercise training appears to maintain telomeres in certain somatic cells, although the underlying molecular mechanisms are incompletely understood. Here, we examined the influence of a single bout of vigorous exercise training on leukocyte telomerase reverse transcriptase (TERT) and shelterin gene expression, and the abundance of three microRNAs (miRNAs) implicated in biological ageing (miRNA-143, -223 and -486-5p) in an elite athlete and large animal model, Thoroughbred horses. Gene and miRNA expression were analysed using primer-based and TaqMan Assay qPCR. Leukocyte TRF1, TRF2 and POT1 expression were all significantly increased whilst miR-223 and miR-486-5p were decreased immediately after vigorous exercise (all p < 0.05), and tended to return to baseline levels 24 h after training. Relative to the young horses (~ 3.9 years old), middle-aged horses (~ 14.8 years old) exhibited reduced leukocyte TERT gene expression, and increased POT1 and miR-223 abundance (all p < 0.05). These data demonstrate that genes transcribing key components of the shelterin-telomere complex are influenced by ageing and dynamically regulated by a single bout of vigorous exercise in a large, athletic mammal — Thoroughbred horses. Our findings also implicate TERT and shelterin gene transcripts as potential targets of miR-223 and miR-486-5p, which are modulated by exercise and may have a role in the telomere maintenance and genomic stability associated with long-term aerobic training. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00424-022-02745-0. Springer Berlin Heidelberg 2022-09-09 2022 /pmc/articles/PMC9560944/ /pubmed/36085194 http://dx.doi.org/10.1007/s00424-022-02745-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Molecular and Genomic Physiology Mandal, Shama Denham, Michele M. Spencer, Sarah J. Denham, Joshua Exercise regulates shelterin genes and microRNAs implicated in ageing in Thoroughbred horses |
title | Exercise regulates shelterin genes and microRNAs implicated in ageing in Thoroughbred horses |
title_full | Exercise regulates shelterin genes and microRNAs implicated in ageing in Thoroughbred horses |
title_fullStr | Exercise regulates shelterin genes and microRNAs implicated in ageing in Thoroughbred horses |
title_full_unstemmed | Exercise regulates shelterin genes and microRNAs implicated in ageing in Thoroughbred horses |
title_short | Exercise regulates shelterin genes and microRNAs implicated in ageing in Thoroughbred horses |
title_sort | exercise regulates shelterin genes and micrornas implicated in ageing in thoroughbred horses |
topic | Molecular and Genomic Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9560944/ https://www.ncbi.nlm.nih.gov/pubmed/36085194 http://dx.doi.org/10.1007/s00424-022-02745-0 |
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