Generation of 2C-like mouse embryonic stem cells in vivo to evaluate developmental potency

This protocol describes an optimized workflow for generating 2-cell (2C) stage-like mouse embryonic stem cells (mESCs) for microinjection into 8-cell stage mouse embryos to evaluate the developmental potency of these cells. We detail the following steps: 1) chemical suppression of glycolysis to indu...

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Detalles Bibliográficos
Autores principales: Tan, Dennis EK, Wu, Baojiang, Bao, Siqin, Tee, Wee-Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562427/
https://www.ncbi.nlm.nih.gov/pubmed/36208454
http://dx.doi.org/10.1016/j.xpro.2022.101684

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562427/
https://www.ncbi.nlm.nih.gov/pubmed/36208454
http://dx.doi.org/10.1016/j.xpro.2022.101684

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