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Generation of 2C-like mouse embryonic stem cells in vivo to evaluate developmental potency
This protocol describes an optimized workflow for generating 2-cell (2C) stage-like mouse embryonic stem cells (mESCs) for microinjection into 8-cell stage mouse embryos to evaluate the developmental potency of these cells. We detail the following steps: 1) chemical suppression of glycolysis to indu...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562427/ https://www.ncbi.nlm.nih.gov/pubmed/36208454 http://dx.doi.org/10.1016/j.xpro.2022.101684 |