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Rapid and accurate quantification of isomiRs by RT-qPCR

Currently, microRNAs (miRs) are annotated as a single defined sequence (canonical), even though high-throughput small RNA sequencing has identified miR isoforms (isomiRs) that differ from their canonical counterparts in length, sequence, or both. Here we describe a simple reverse transcriptase-quant...

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Detalles Bibliográficos
Autores principales: Franco, Sandra, Pluvinet, Raquel, Sanchez-Herrero, Jose Francisco, Sumoy, Lauro, Martinez, Miguel Angel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9568571/
https://www.ncbi.nlm.nih.gov/pubmed/36241713
http://dx.doi.org/10.1038/s41598-022-22298-7