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Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromes

Drug testing assays in hematopoietic stem and progenitor cells (HSPCs) are fundamental in biological studies of myelodysplastic syndromes (MDS) but have historically entailed a technical challenge. This protocol allows the efficient isolation of MDS HSPCs from bone marrow mononuclear cell fractions...

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Detalles Bibliográficos
Autores principales: Ganan-Gomez, Irene, Clise-Dwyer, Karen, Colla, Simona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9568884/
https://www.ncbi.nlm.nih.gov/pubmed/36240061
http://dx.doi.org/10.1016/j.xpro.2022.101764