Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromes

Drug testing assays in hematopoietic stem and progenitor cells (HSPCs) are fundamental in biological studies of myelodysplastic syndromes (MDS) but have historically entailed a technical challenge. This protocol allows the efficient isolation of MDS HSPCs from bone marrow mononuclear cell fractions...

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Autores principales: Ganan-Gomez, Irene, Clise-Dwyer, Karen, Colla, Simona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9568884/
https://www.ncbi.nlm.nih.gov/pubmed/36240061
http://dx.doi.org/10.1016/j.xpro.2022.101764
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author Ganan-Gomez, Irene
Clise-Dwyer, Karen
Colla, Simona
author_facet Ganan-Gomez, Irene
Clise-Dwyer, Karen
Colla, Simona
author_sort Ganan-Gomez, Irene
collection PubMed
description Drug testing assays in hematopoietic stem and progenitor cells (HSPCs) are fundamental in biological studies of myelodysplastic syndromes (MDS) but have historically entailed a technical challenge. This protocol allows the efficient isolation of MDS HSPCs from bone marrow mononuclear cell fractions and their culturing with the support of stromal cells for improved maintenance during drug testing. Lastly, specific steps are given to quantify surviving cells and assess changes in the HSPC hierarchies. For complete details on the use and execution of this protocol, please refer to Ganan-Gomez et al. (2022).
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spelling pubmed-95688842022-10-16 Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromes Ganan-Gomez, Irene Clise-Dwyer, Karen Colla, Simona STAR Protoc Protocol Drug testing assays in hematopoietic stem and progenitor cells (HSPCs) are fundamental in biological studies of myelodysplastic syndromes (MDS) but have historically entailed a technical challenge. This protocol allows the efficient isolation of MDS HSPCs from bone marrow mononuclear cell fractions and their culturing with the support of stromal cells for improved maintenance during drug testing. Lastly, specific steps are given to quantify surviving cells and assess changes in the HSPC hierarchies. For complete details on the use and execution of this protocol, please refer to Ganan-Gomez et al. (2022). Elsevier 2022-10-13 /pmc/articles/PMC9568884/ /pubmed/36240061 http://dx.doi.org/10.1016/j.xpro.2022.101764 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Ganan-Gomez, Irene
Clise-Dwyer, Karen
Colla, Simona
Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromes
title Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromes
title_full Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromes
title_fullStr Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromes
title_full_unstemmed Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromes
title_short Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromes
title_sort isolation, culture, and immunophenotypic analysis of bone marrow hspcs from patients with myelodysplastic syndromes
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9568884/
https://www.ncbi.nlm.nih.gov/pubmed/36240061
http://dx.doi.org/10.1016/j.xpro.2022.101764
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