mRNA Capture Sequencing and RT-qPCR for the Detection of Pathognomonic, Novel, and Secondary Fusion Transcripts in FFPE Tissue: A Sarcoma Showcase

We assess the performance of mRNA capture sequencing to identify fusion transcripts in FFPE tissue of different sarcoma types, followed by RT-qPCR confirmation. To validate our workflow, six positive control tumors with a specific chromosomal rearrangement were analyzed using the TruSight RNA Pan-Ca...

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Detalles Bibliográficos
Autores principales: Decock, Anneleen, Creytens, David, Lefever, Steve, Van der Meulen, Joni, Anckaert, Jasper, De Ganck, Ariane, Deleu, Jill, De Wilde, Bram, Fierro, Carolina, Kuersten, Scott, Luypaert, Manuel, Rottiers, Isabelle, Schroth, Gary P., Steyaert, Sandra, Vanderheyden, Katrien, Vanden Eynde, Eveline, Verniers, Kimberly, Verreth, Joke, Van Dorpe, Jo, Vandesompele, Jo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9569610/
https://www.ncbi.nlm.nih.gov/pubmed/36232302
http://dx.doi.org/10.3390/ijms231911007

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9569610/
https://www.ncbi.nlm.nih.gov/pubmed/36232302
http://dx.doi.org/10.3390/ijms231911007

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