Generation of Flag/DYKDDDDK Epitope Tag Knock-In Mice Using i-GONAD Enables Detection of Endogenous CaMKIIα and β Proteins

Specific antibodies are necessary for cellular and tissue expression, biochemical, and functional analyses of protein complexes. However, generating a specific antibody is often time-consuming and effort-intensive. The epitope tagging of an endogenous protein at an appropriate position can overcome...

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Detalles Bibliográficos
Autores principales: Aoto, Kazushi, Takabayashi, Shuji, Mutoh, Hiroki, Saitsu, Hirotomo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9569722/
https://www.ncbi.nlm.nih.gov/pubmed/36233218
http://dx.doi.org/10.3390/ijms231911915

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9569722/
https://www.ncbi.nlm.nih.gov/pubmed/36233218
http://dx.doi.org/10.3390/ijms231911915

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