Cargando…

Methodological and Biological Factors Influencing Global DNA Methylation Results Measured by LINE-1 Pyrosequencing Assay in Colorectal Tissue and Liquid Biopsy Samples

Long interspersed nuclear element 1 (LINE-1) bisulfite pyrosequencing is a widely used technique for genome-wide methylation analyses. We aimed to investigate the effects of experimental and biological factors on its results to improve the comparability. LINE-1 bisulfite pyrosequencing was performed...

Descripción completa

Detalles Bibliográficos
Autores principales:	Szigeti, Krisztina A, Barták, Barbara K, Nagy, Zsófia B, Zsigrai, Sára, Papp, Márton, Márkus, Eszter, Igaz, Peter, Takács, István, Molnár, Béla, Kalmár, Alexandra
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9569782/ https://www.ncbi.nlm.nih.gov/pubmed/36232908 http://dx.doi.org/10.3390/ijms231911608

Ejemplares similares

A Liquid Biopsy-Based Approach for Monitoring Treatment Response in Post-Operative Colorectal Cancer Patients
por: Barták, Barbara Kinga, et al.
Publicado: (2022)

Global DNA hypomethylation of colorectal tumours detected in tissue and liquid biopsies may be related to decreased methyl-donor content
por: Szigeti, Krisztina A., et al.
Publicado: (2022)

A Detailed Overview About the Single-Cell Analyses of Solid Tumors Focusing on Colorectal Cancer
por: Kothalawala, William J., et al.
Publicado: (2022)

Patterns of Somatic Variants in Colorectal Adenoma and Carcinoma Tissue and Matched Plasma Samples from the Hungarian Oncogenome Program
por: Kalmár, Alexandra, et al.
Publicado: (2023)

Folic Acid Treatment Directly Influences the Genetic and Epigenetic Regulation along with the Associated Cellular Maintenance Processes of HT-29 and SW480 Colorectal Cancer Cell Lines
por: Zsigrai, Sára, et al.
Publicado: (2022)

Diagnostic and prognostic potential of tissue and circulating long non-coding RNAs in colorectal tumors
por: Galamb, Orsolya, et al.
Publicado: (2019)

S-Adenosylmethionine Treatment of Colorectal Cancer Cell Lines Alters DNA Methylation, DNA Repair and Tumor Progression-Related Gene Expression
por: Zsigrai, Sára, et al.
Publicado: (2020)

Gene promoter and exon DNA methylation changes in colon cancer development – mRNA expression and tumor mutation alterations
por: Molnár, Béla, et al.
Publicado: (2018)

Promoter Hypomethylation and Increased Expression of the Long Non-coding RNA LINC00152 Support Colorectal Carcinogenesis
por: Galamb, Orsolya, et al.
Publicado: (2020)

Colorectal adenoma and carcinoma specific miRNA profiles in biopsy and their expression in plasma specimens
por: Nagy, Zsófia Brigitta, et al.
Publicado: (2017)

Genome-wide expression profiling in colorectal cancer focusing on lncRNAs in the adenoma-carcinoma transition
por: Kalmár, Alexandra, et al.
Publicado: (2019)

Cell Free DNA of Tumor Origin Induces a ‘Metastatic’ Expression Profile in HT-29 Cancer Cell Line
por: Fűri, István, et al.
Publicado: (2015)

New Insights into the Methodology of L-Arginine-Induced Acute Pancreatitis
por: Kui, Balázs, et al.
Publicado: (2015)

En bloc release of MVB-like small extracellular vesicle clusters by colorectal carcinoma cells
por: Valcz, Gábor, et al.
Publicado: (2019)

The Effectiveness of Psychological Interventions for Rheumatoid Arthritis (RA): A Systematic Review and Meta-Analysis
por: Nagy, Zsófia, et al.
Publicado: (2023)

Development of a pyrosequencing assay for the typing of alphaherpesviruses()
por: Fusco, G., et al.
Publicado: (2015)

Liquid biopsy for the assessment of adrenal cancer heterogeneity: where do we stand?
por: Perge, Pál, et al.
Publicado: (2022)

Small extracellular vesicle DNA-mediated horizontal gene transfer as a driving force for tumor evolution: Facts and riddles
por: Valcz, Gábor, et al.
Publicado: (2022)

Perspective: bidirectional exosomal transport between cancer stem cells and their fibroblast-rich microenvironment during metastasis formation
por: Valcz, Gábor, et al.
Publicado: (2018)

Liquid Biopsy to Detect Minimal Residual Disease: Methodology and Impact
por: Honoré, Natasha, et al.
Publicado: (2021)

mPSQed: A Software for the Design of Multiplex Pyrosequencing Assays
por: Dabrowski, Piotr Wojtek, et al.
Publicado: (2012)

Pyrosequencing assay for rapid identification of Mycobacterium tuberculosis complex species
por: Kahla, Imen Ben, et al.
Publicado: (2011)

Dysplasia-Carcinoma Transition Specific Transcripts in Colonic Biopsy Samples
por: Galamb, Orsolya, et al.
Publicado: (2012)

Folate-Targeted Monodisperse PEG-Based Conjugates Made by Chemo-Enzymatic Methods for Cancer Diagnosis and Treatment
por: Nagy, Krisztina S., et al.
Publicado: (2021)

New methodology of TMB assessment from tissue and liquid biopsy in NSCLC
por: Křížová, Ľudmila, et al.
Publicado: (2022)

Gene-expression analysis of a colorectal cancer-specific discriminatory transcript set on formalin-fixed, paraffin-embedded (FFPE) tissue samples
por: Kalmár, Alexandra, et al.
Publicado: (2015)

Biofluids manipulation methods for liquid biopsy in minimally-invasive assays
por: Garzarelli, Valeria, et al.
Publicado: (2022)

Water-Ionic Liquid Binary Mixture Tailored Resorcinol-Formaldehyde Carbon Aerogels without Added Catalyst
por: Nagy, Balázs, et al.
Publicado: (2019)

Methylation enrichment pyrosequencing: combining the specificity of MSP with validation by pyrosequencing
por: Shaw, Richard J., et al.
Publicado: (2006)

Chemical Oscillations With Sodium Perborate as Oxidant
por: Kurin-Csörgei, Krisztina, et al.
Publicado: (2020)

Oral Microbiome Profiles: 16S rRNA Pyrosequencing and Microarray Assay Comparison
por: Ahn, Jiyoung, et al.
Publicado: (2011)

Shedding light on the performance of a pyrosequencing assay for drug-resistant tuberculosis diagnosis
por: Georghiou, Sophia B., et al.
Publicado: (2016)

PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping
por: Molina-Moya, B., et al.
Publicado: (2017)

Liquid biopsy assay for pulmonary adenocarcinoma using supernatants from core‐needle biopsy specimens
por: Kong, Fanlei, et al.
Publicado: (2022)

Aging related methylation influences the gene expression of key control genes in colorectal cancer and adenoma
por: Galamb, Orsolya, et al.
Publicado: (2016)

Appearance of Thiacloprid in the Guttation Liquid of Coated Maize Seeds
por: Mörtl, Mária, et al.
Publicado: (2020)

Construction of a multiplex mutation hot spot PCR panel: the first step towards colorectal cancer genotyping on the GS Junior platform
por: Péterfia, Bálint, et al.
Publicado: (2017)

Liquid biopsy-based monitoring of residual disease in multiple myeloma by analysis of the rearranged immunoglobulin genes–A feasibility study
por: Marx, Anita, et al.
Publicado: (2023)

Pyrosequencing Bacillus anthracis
por: Wahab, Tara, et al.
Publicado: (2005)

Liquid Biopsies: Applications for Cancer Diagnosis and Monitoring
por: Martins, Ivana, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_f9bcjfan59c8kegcrf3uk3gf51