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Chromone-Containing Allylmorpholines Influence Ion Channels in Lipid Membranes via Dipole Potential and Packing Stress

Herein, we report that chromone-containing allylmorpholines can affect ion channels formed by pore-forming antibiotics in model lipid membranes, which correlates with their ability to influence membrane boundary potential and lipid-packing stress. At 100 µg/mL, allylmorpholines 1, 6, 7, and 8 decrea...

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Autores principales: Efimova, Svetlana S., Martynyuk, Vera A., Zakharova, Anastasiia A., Yudintceva, Natalia M., Chernov, Nikita M., Yakovlev, Igor P., Ostroumova, Olga S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570167/
https://www.ncbi.nlm.nih.gov/pubmed/36232854
http://dx.doi.org/10.3390/ijms231911554
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author Efimova, Svetlana S.
Martynyuk, Vera A.
Zakharova, Anastasiia A.
Yudintceva, Natalia M.
Chernov, Nikita M.
Yakovlev, Igor P.
Ostroumova, Olga S.
author_facet Efimova, Svetlana S.
Martynyuk, Vera A.
Zakharova, Anastasiia A.
Yudintceva, Natalia M.
Chernov, Nikita M.
Yakovlev, Igor P.
Ostroumova, Olga S.
author_sort Efimova, Svetlana S.
collection PubMed
description Herein, we report that chromone-containing allylmorpholines can affect ion channels formed by pore-forming antibiotics in model lipid membranes, which correlates with their ability to influence membrane boundary potential and lipid-packing stress. At 100 µg/mL, allylmorpholines 1, 6, 7, and 8 decrease the boundary potential of the bilayers composed of palmitoyloleoylphosphocholine (POPC) by about 100 mV. At the same time, the compounds do not affect the zeta-potential of POPC liposomes, but reduce the membrane dipole potential by 80–120 mV. The allylmorpholine-induced drop in the dipole potential produce 10–30% enhancement in the conductance of gramicidin A channels. Chromone-containing allylmorpholines also affect the thermotropic behavior of dipalmytoylphosphocholine (DPPC), abolishing the pretransition, lowering melting cooperativity, and turning the main phase transition peak into a multicomponent profile. Compounds 4, 6, 7, and 8 are able to decrease DPPC’s melting temperature by about 0.5–1.9 °C. Moreover, derivative 7 is shown to increase the temperature of transition of palmitoyloleoylphosphoethanolamine from lamellar to inverted hexagonal phase. The effects on lipid-phase transitions are attributed to the changes in the spontaneous curvature stress. Alterations in lipid packing induced by allylmorpholines are believed to potentiate the pore-forming ability of amphotericin B and gramicidin A by several times.
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spelling pubmed-95701672022-10-17 Chromone-Containing Allylmorpholines Influence Ion Channels in Lipid Membranes via Dipole Potential and Packing Stress Efimova, Svetlana S. Martynyuk, Vera A. Zakharova, Anastasiia A. Yudintceva, Natalia M. Chernov, Nikita M. Yakovlev, Igor P. Ostroumova, Olga S. Int J Mol Sci Article Herein, we report that chromone-containing allylmorpholines can affect ion channels formed by pore-forming antibiotics in model lipid membranes, which correlates with their ability to influence membrane boundary potential and lipid-packing stress. At 100 µg/mL, allylmorpholines 1, 6, 7, and 8 decrease the boundary potential of the bilayers composed of palmitoyloleoylphosphocholine (POPC) by about 100 mV. At the same time, the compounds do not affect the zeta-potential of POPC liposomes, but reduce the membrane dipole potential by 80–120 mV. The allylmorpholine-induced drop in the dipole potential produce 10–30% enhancement in the conductance of gramicidin A channels. Chromone-containing allylmorpholines also affect the thermotropic behavior of dipalmytoylphosphocholine (DPPC), abolishing the pretransition, lowering melting cooperativity, and turning the main phase transition peak into a multicomponent profile. Compounds 4, 6, 7, and 8 are able to decrease DPPC’s melting temperature by about 0.5–1.9 °C. Moreover, derivative 7 is shown to increase the temperature of transition of palmitoyloleoylphosphoethanolamine from lamellar to inverted hexagonal phase. The effects on lipid-phase transitions are attributed to the changes in the spontaneous curvature stress. Alterations in lipid packing induced by allylmorpholines are believed to potentiate the pore-forming ability of amphotericin B and gramicidin A by several times. MDPI 2022-09-30 /pmc/articles/PMC9570167/ /pubmed/36232854 http://dx.doi.org/10.3390/ijms231911554 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Efimova, Svetlana S.
Martynyuk, Vera A.
Zakharova, Anastasiia A.
Yudintceva, Natalia M.
Chernov, Nikita M.
Yakovlev, Igor P.
Ostroumova, Olga S.
Chromone-Containing Allylmorpholines Influence Ion Channels in Lipid Membranes via Dipole Potential and Packing Stress
title Chromone-Containing Allylmorpholines Influence Ion Channels in Lipid Membranes via Dipole Potential and Packing Stress
title_full Chromone-Containing Allylmorpholines Influence Ion Channels in Lipid Membranes via Dipole Potential and Packing Stress
title_fullStr Chromone-Containing Allylmorpholines Influence Ion Channels in Lipid Membranes via Dipole Potential and Packing Stress
title_full_unstemmed Chromone-Containing Allylmorpholines Influence Ion Channels in Lipid Membranes via Dipole Potential and Packing Stress
title_short Chromone-Containing Allylmorpholines Influence Ion Channels in Lipid Membranes via Dipole Potential and Packing Stress
title_sort chromone-containing allylmorpholines influence ion channels in lipid membranes via dipole potential and packing stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570167/
https://www.ncbi.nlm.nih.gov/pubmed/36232854
http://dx.doi.org/10.3390/ijms231911554
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