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Tryptamine, a Microbial Metabolite in Fermented Rice Bran Suppressed Lipopolysaccharide-Induced Inflammation in a Murine Macrophage Model
Fermentation is thought to alter the composition and bioavailability of bioactive compounds in rice bran. However, how this process affects the anti-inflammatory effects of rice bran and the bioactive compounds that might participate in this function is yet to be elucidated. This study aimed to isol...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570467/ https://www.ncbi.nlm.nih.gov/pubmed/36232510 http://dx.doi.org/10.3390/ijms231911209 |
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author | Agista, Afifah Zahra Tanuseputero, Sharon Angela Koseki, Takuya , Ardiansyah Budijanto, Slamet Sultana, Halima Ohsaki, Yusuke Yeh, Chiu-Li Yang, Suh-Ching Komai, Michio Shirakawa, Hitoshi |
author_facet | Agista, Afifah Zahra Tanuseputero, Sharon Angela Koseki, Takuya , Ardiansyah Budijanto, Slamet Sultana, Halima Ohsaki, Yusuke Yeh, Chiu-Li Yang, Suh-Ching Komai, Michio Shirakawa, Hitoshi |
author_sort | Agista, Afifah Zahra |
collection | PubMed |
description | Fermentation is thought to alter the composition and bioavailability of bioactive compounds in rice bran. However, how this process affects the anti-inflammatory effects of rice bran and the bioactive compounds that might participate in this function is yet to be elucidated. This study aimed to isolate bioactive compounds in fermented rice bran that play a key role in its anti-inflammatory function. The fermented rice bran was fractionated using a succession of solvent and solid-phase extractions. The fermented rice bran fractions were then applied to lipopolysaccharide (LPS)-activated murine macrophages to evaluate their anti-inflammatory activity. The hot water fractions (FRBA), 50% ethanol fractions (FRBB), and n-hexane fractions (FRBC) were all shown to be able to suppress the pro-inflammatory cytokine expression from LPS-stimulated RAW 264.7 cells. Subsequent fractions from the hot water fraction (FRBF and FRBE) were also able to reduce the inflammatory response of these cells to LPS. Further investigation revealed that tryptamine, a bacterial metabolite of tryptophan, was abundantly present in these extracts. These results indicate that tryptamine may play an important role in the anti-inflammatory effects of fermented rice bran. Furthermore, the anti-inflammatory effects of FRBE and tryptamine may depend on the activity of the aryl hydrocarbon receptor. |
format | Online Article Text |
id | pubmed-9570467 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95704672022-10-17 Tryptamine, a Microbial Metabolite in Fermented Rice Bran Suppressed Lipopolysaccharide-Induced Inflammation in a Murine Macrophage Model Agista, Afifah Zahra Tanuseputero, Sharon Angela Koseki, Takuya , Ardiansyah Budijanto, Slamet Sultana, Halima Ohsaki, Yusuke Yeh, Chiu-Li Yang, Suh-Ching Komai, Michio Shirakawa, Hitoshi Int J Mol Sci Article Fermentation is thought to alter the composition and bioavailability of bioactive compounds in rice bran. However, how this process affects the anti-inflammatory effects of rice bran and the bioactive compounds that might participate in this function is yet to be elucidated. This study aimed to isolate bioactive compounds in fermented rice bran that play a key role in its anti-inflammatory function. The fermented rice bran was fractionated using a succession of solvent and solid-phase extractions. The fermented rice bran fractions were then applied to lipopolysaccharide (LPS)-activated murine macrophages to evaluate their anti-inflammatory activity. The hot water fractions (FRBA), 50% ethanol fractions (FRBB), and n-hexane fractions (FRBC) were all shown to be able to suppress the pro-inflammatory cytokine expression from LPS-stimulated RAW 264.7 cells. Subsequent fractions from the hot water fraction (FRBF and FRBE) were also able to reduce the inflammatory response of these cells to LPS. Further investigation revealed that tryptamine, a bacterial metabolite of tryptophan, was abundantly present in these extracts. These results indicate that tryptamine may play an important role in the anti-inflammatory effects of fermented rice bran. Furthermore, the anti-inflammatory effects of FRBE and tryptamine may depend on the activity of the aryl hydrocarbon receptor. MDPI 2022-09-23 /pmc/articles/PMC9570467/ /pubmed/36232510 http://dx.doi.org/10.3390/ijms231911209 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Agista, Afifah Zahra Tanuseputero, Sharon Angela Koseki, Takuya , Ardiansyah Budijanto, Slamet Sultana, Halima Ohsaki, Yusuke Yeh, Chiu-Li Yang, Suh-Ching Komai, Michio Shirakawa, Hitoshi Tryptamine, a Microbial Metabolite in Fermented Rice Bran Suppressed Lipopolysaccharide-Induced Inflammation in a Murine Macrophage Model |
title | Tryptamine, a Microbial Metabolite in Fermented Rice Bran Suppressed Lipopolysaccharide-Induced Inflammation in a Murine Macrophage Model |
title_full | Tryptamine, a Microbial Metabolite in Fermented Rice Bran Suppressed Lipopolysaccharide-Induced Inflammation in a Murine Macrophage Model |
title_fullStr | Tryptamine, a Microbial Metabolite in Fermented Rice Bran Suppressed Lipopolysaccharide-Induced Inflammation in a Murine Macrophage Model |
title_full_unstemmed | Tryptamine, a Microbial Metabolite in Fermented Rice Bran Suppressed Lipopolysaccharide-Induced Inflammation in a Murine Macrophage Model |
title_short | Tryptamine, a Microbial Metabolite in Fermented Rice Bran Suppressed Lipopolysaccharide-Induced Inflammation in a Murine Macrophage Model |
title_sort | tryptamine, a microbial metabolite in fermented rice bran suppressed lipopolysaccharide-induced inflammation in a murine macrophage model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570467/ https://www.ncbi.nlm.nih.gov/pubmed/36232510 http://dx.doi.org/10.3390/ijms231911209 |
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