Pipeline for fluorescent imaging and volumetric analysis of neurons in cleared mouse spinal cords

Precisely measuring the number and somatic volume of neurons in the central nervous system at single-cell resolution is technically challenging. Here, we combine multiple techniques to address this challenge in optically cleared mouse spinal cords. We describe in vivo neuron labeling approaches, tis...

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Detalles Bibliográficos
Autores principales: Yu, Hao, Li, Qiang, Sandoval, Alfredo, Gibbs, Holly C., English, Amber, Dunn, Tiffany, Moth, John, Elahi, Hajira, Chen, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9576748/
https://www.ncbi.nlm.nih.gov/pubmed/36227743
http://dx.doi.org/10.1016/j.xpro.2022.101759

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9576748/
https://www.ncbi.nlm.nih.gov/pubmed/36227743
http://dx.doi.org/10.1016/j.xpro.2022.101759

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