Pipeline for fluorescent imaging and volumetric analysis of neurons in cleared mouse spinal cords

Precisely measuring the number and somatic volume of neurons in the central nervous system at single-cell resolution is technically challenging. Here, we combine multiple techniques to address this challenge in optically cleared mouse spinal cords. We describe in vivo neuron labeling approaches, tis...

Descripción completa

Detalles Bibliográficos
Autores principales: Yu, Hao, Li, Qiang, Sandoval, Alfredo, Gibbs, Holly C., English, Amber, Dunn, Tiffany, Moth, John, Elahi, Hajira, Chen, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9576748/
https://www.ncbi.nlm.nih.gov/pubmed/36227743
http://dx.doi.org/10.1016/j.xpro.2022.101759

Ejemplares similares

Ejemplares similares