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Loss-of-function of triacylglycerol lipases are associated with low flour rancidity in pearl millet [Pennisetum glaucum (L.) R. Br.]

Pearl millet is an important cereal crop of semi-arid regions since it is highly nutritious and climate resilient. However, pearl millet is underutilized commercially due to the rapid onset of hydrolytic rancidity of seed lipids post-milling. We investigated the underlying biochemical and molecular...

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Autores principales: Aher, Rasika Rajendra, Reddy, Palakolanu Sudhakar, Bhunia, Rupam Kumar, Flyckt, Kayla S., Shankhapal, Aishwarya R., Ojha, Rabishankar, Everard, John D., Wayne, Laura L., Ruddy, Brian M., Deonovic, Benjamin, Gupta, Shashi K., Sharma, Kiran K., Bhatnagar-Mathur, Pooja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9577237/
https://www.ncbi.nlm.nih.gov/pubmed/36267938
http://dx.doi.org/10.3389/fpls.2022.962667
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author Aher, Rasika Rajendra
Reddy, Palakolanu Sudhakar
Bhunia, Rupam Kumar
Flyckt, Kayla S.
Shankhapal, Aishwarya R.
Ojha, Rabishankar
Everard, John D.
Wayne, Laura L.
Ruddy, Brian M.
Deonovic, Benjamin
Gupta, Shashi K.
Sharma, Kiran K.
Bhatnagar-Mathur, Pooja
author_facet Aher, Rasika Rajendra
Reddy, Palakolanu Sudhakar
Bhunia, Rupam Kumar
Flyckt, Kayla S.
Shankhapal, Aishwarya R.
Ojha, Rabishankar
Everard, John D.
Wayne, Laura L.
Ruddy, Brian M.
Deonovic, Benjamin
Gupta, Shashi K.
Sharma, Kiran K.
Bhatnagar-Mathur, Pooja
author_sort Aher, Rasika Rajendra
collection PubMed
description Pearl millet is an important cereal crop of semi-arid regions since it is highly nutritious and climate resilient. However, pearl millet is underutilized commercially due to the rapid onset of hydrolytic rancidity of seed lipids post-milling. We investigated the underlying biochemical and molecular mechanisms of rancidity development in the flour from contrasting inbred lines under accelerated aging conditions. The breakdown of storage lipids (triacylglycerols; TAG) was accompanied by free fatty acid accumulation over the time course for all lines. The high rancidity lines had the highest amount of FFA by day 21, suggesting that TAG lipases may be the cause of rancidity. Additionally, the high rancidity lines manifested substantial amounts of volatile aldehyde compounds, which are characteristic products of lipid oxidation. Lipases with expression in seed post-milling were sequenced from low and high rancidity lines. Polymorphisms were identified in two TAG lipase genes (PgTAGLip1 and PgTAGLip2) from the low rancidity line. Expression in a yeast model system confirmed these mutants were non-functional. We provide a direct mechanism to alleviate rancidity in pearl millet flour by identifying mutations in key TAG lipase genes that are associated with low rancidity. These genetic variations can be exploited through molecular breeding or precision genome technologies to develop elite pearl millet cultivars with improved flour shelf life.
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spelling pubmed-95772372022-10-19 Loss-of-function of triacylglycerol lipases are associated with low flour rancidity in pearl millet [Pennisetum glaucum (L.) R. Br.] Aher, Rasika Rajendra Reddy, Palakolanu Sudhakar Bhunia, Rupam Kumar Flyckt, Kayla S. Shankhapal, Aishwarya R. Ojha, Rabishankar Everard, John D. Wayne, Laura L. Ruddy, Brian M. Deonovic, Benjamin Gupta, Shashi K. Sharma, Kiran K. Bhatnagar-Mathur, Pooja Front Plant Sci Plant Science Pearl millet is an important cereal crop of semi-arid regions since it is highly nutritious and climate resilient. However, pearl millet is underutilized commercially due to the rapid onset of hydrolytic rancidity of seed lipids post-milling. We investigated the underlying biochemical and molecular mechanisms of rancidity development in the flour from contrasting inbred lines under accelerated aging conditions. The breakdown of storage lipids (triacylglycerols; TAG) was accompanied by free fatty acid accumulation over the time course for all lines. The high rancidity lines had the highest amount of FFA by day 21, suggesting that TAG lipases may be the cause of rancidity. Additionally, the high rancidity lines manifested substantial amounts of volatile aldehyde compounds, which are characteristic products of lipid oxidation. Lipases with expression in seed post-milling were sequenced from low and high rancidity lines. Polymorphisms were identified in two TAG lipase genes (PgTAGLip1 and PgTAGLip2) from the low rancidity line. Expression in a yeast model system confirmed these mutants were non-functional. We provide a direct mechanism to alleviate rancidity in pearl millet flour by identifying mutations in key TAG lipase genes that are associated with low rancidity. These genetic variations can be exploited through molecular breeding or precision genome technologies to develop elite pearl millet cultivars with improved flour shelf life. Frontiers Media S.A. 2022-10-04 /pmc/articles/PMC9577237/ /pubmed/36267938 http://dx.doi.org/10.3389/fpls.2022.962667 Text en Copyright © 2022 Aher, Reddy, Bhunia, Flyckt, Shankhapal, Ojha, Everard, Wayne, Ruddy, Deonovic, Gupta, Sharma and Bhatnagar-Mathur. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Aher, Rasika Rajendra
Reddy, Palakolanu Sudhakar
Bhunia, Rupam Kumar
Flyckt, Kayla S.
Shankhapal, Aishwarya R.
Ojha, Rabishankar
Everard, John D.
Wayne, Laura L.
Ruddy, Brian M.
Deonovic, Benjamin
Gupta, Shashi K.
Sharma, Kiran K.
Bhatnagar-Mathur, Pooja
Loss-of-function of triacylglycerol lipases are associated with low flour rancidity in pearl millet [Pennisetum glaucum (L.) R. Br.]
title Loss-of-function of triacylglycerol lipases are associated with low flour rancidity in pearl millet [Pennisetum glaucum (L.) R. Br.]
title_full Loss-of-function of triacylglycerol lipases are associated with low flour rancidity in pearl millet [Pennisetum glaucum (L.) R. Br.]
title_fullStr Loss-of-function of triacylglycerol lipases are associated with low flour rancidity in pearl millet [Pennisetum glaucum (L.) R. Br.]
title_full_unstemmed Loss-of-function of triacylglycerol lipases are associated with low flour rancidity in pearl millet [Pennisetum glaucum (L.) R. Br.]
title_short Loss-of-function of triacylglycerol lipases are associated with low flour rancidity in pearl millet [Pennisetum glaucum (L.) R. Br.]
title_sort loss-of-function of triacylglycerol lipases are associated with low flour rancidity in pearl millet [pennisetum glaucum (l.) r. br.]
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9577237/
https://www.ncbi.nlm.nih.gov/pubmed/36267938
http://dx.doi.org/10.3389/fpls.2022.962667
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