Agarose gel microcapsules enable easy-to-prepare, picolitre-scale, single-cell genomics, yielding high-coverage genome sequences

A novel type of agarose gel microcapsule (AGM), consisting of an alginate picolitre sol core and an agarose gel shell, was developed to obtain high-quality, single-cell, amplified genomic DNA of bacteria. The AGM is easy to prepare in a stable emulsion with oil of water-equivalent density, which pre...

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Detalles Bibliográficos
Autores principales: Aoki, Hiroyoshi, Masahiro, Yuki, Shimizu, Michiru, Hongoh, Yuichi, Ohkuma, Moriya, Yamagata, Yutaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9579161/
https://www.ncbi.nlm.nih.gov/pubmed/36257967
http://dx.doi.org/10.1038/s41598-022-20923-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9579161/
https://www.ncbi.nlm.nih.gov/pubmed/36257967
http://dx.doi.org/10.1038/s41598-022-20923-z

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