Protocol for rapid and accurate quantification of phospholipids in yeast and mammalian systems using LC-MS

We describe a protocol for measuring phospholipid class and fatty acid composition in the budding yeast Saccharomyces cerevisiae using a liquid chromatography-mass spectrometry (LC-MS)-based approach. We compile a mass spectral-retention time library verified for major phospholipids in the budding y...

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Detalles Bibliográficos
Autores principales: Yang, Sen, Xue, Jingyuan, Ye, Cunqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9579711/
https://www.ncbi.nlm.nih.gov/pubmed/36240059
http://dx.doi.org/10.1016/j.xpro.2022.101769

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9579711/
https://www.ncbi.nlm.nih.gov/pubmed/36240059
http://dx.doi.org/10.1016/j.xpro.2022.101769

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