Cargando…

Protocol for rapid and accurate quantification of phospholipids in yeast and mammalian systems using LC-MS

We describe a protocol for measuring phospholipid class and fatty acid composition in the budding yeast Saccharomyces cerevisiae using a liquid chromatography-mass spectrometry (LC-MS)-based approach. We compile a mass spectral-retention time library verified for major phospholipids in the budding y...

Descripción completa

Detalles Bibliográficos
Autores principales:	Yang, Sen, Xue, Jingyuan, Ye, Cunqi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9579711/ https://www.ncbi.nlm.nih.gov/pubmed/36240059 http://dx.doi.org/10.1016/j.xpro.2022.101769

Ejemplares similares

A Fast and Accurate Method to Identify and Quantify Enzymes in Brush-Border Membranes: In Situ Hydrolysis Followed by Nano LC-MS/MS
por: Brun, Antonio, et al.
Publicado: (2020)

Protocol to purify and detect ubiquitinated phospholipids in budding yeast and human cell lines
por: Sakamaki, Jun-ichi, et al.
Publicado: (2022)

MRCQuant- an accurate LC-MS relative isotopic quantification algorithm on TOF instruments
por: Haskins, William E, et al.
Publicado: (2011)

Rapid LC-MS/MS Bosutinib Quantification with Applications in Metabolic Stability Estimation
por: Attwa, Mohamed W., et al.
Publicado: (2023)

Protocol for quantification of the lysosomal degradation of extracellular proteins into mammalian cells
por: Tomihari, Ayaka, et al.
Publicado: (2021)

Quantifying phosphorylation dynamics in primary neuronal cultures using LC-MS/MS
por: Desch, Kristina, et al.
Publicado: (2021)

Rapid and Serial Quantification of Adhesion Forces of Yeast and Mammalian Cells
por: Potthoff, Eva, et al.
Publicado: (2012)

Protocol for global proteome, virome, and metaproteome profiling of respiratory specimen (VTM) in COVID-19 patient by LC-MS/MS-based analysis
por: Tripathi, Gaurav, et al.
Publicado: (2021)

Rapid quantification of fatty acids in plant oils and biological samples by LC-MS
por: Koch, Elisabeth, et al.
Publicado: (2021)

Organoid Sample Preparation and Extraction for LC-MS Peptidomics
por: Miedzybrodzka, Emily L., et al.
Publicado: (2020)

Pipelines and Systems for Threshold-Avoiding Quantification of LC–MS/MS Data
por: Sánchez Brotons, Alejandro, et al.
Publicado: (2021)

Sample preparation for proteomic analysis using a GeLC-MS/MS strategy
por: Paulo, Joao A.
Publicado: (2016)

Optimization of the CYP inhibition assay using LC-MS/MS
por: Abduraman, Muhammad Asyraf, et al.
Publicado: (2022)

Importance of highly selective LC–MS/MS analysis for the accurate quantification of tamoxifen and its metabolites: focus on endoxifen and 4-hydroxytamoxifen
por: Jager, N. G. L., et al.
Publicado: (2012)

Stoichiometric Quantification of Akt Phosphorylation Using LC-MS/MS
por: Atrih, Abdelmadjid, et al.
Publicado: (2009)

An LC–MS Assay to Measure Superoxide Radicals and Hydrogen Peroxide in the Blood System
por: Tsamesidis, Ioannis, et al.
Publicado: (2020)

Targeted LC-MS/MS-based metabolomics and lipidomics on limited hematopoietic stem cell numbers
por: Schönberger, Katharina, et al.
Publicado: (2022)

“Notame”: Workflow for Non-Targeted LC–MS Metabolic Profiling
por: Klåvus, Anton, et al.
Publicado: (2020)

Rapid LC–MS assay for targeted metabolite quantification by serial injection into isocratic gradients
por: Groves, Ryan A., et al.
Publicado: (2022)

A Simple and Rapid LC-MS/MS Method for Quantification of Total Daidzein, Genistein, and Equol in Human Urine
por: Saha, Shikha, et al.
Publicado: (2020)

Nano LC-MS using capillary columns enables accurate quantification of modified ribonucleosides at low femtomol levels
por: Sarin, L. Peter, et al.
Publicado: (2018)

Quantitative analysis of m(6)A RNA modification by LC-MS
por: Mathur, Lavina, et al.
Publicado: (2021)

Estimation of measurement uncertainty for the quantification of protein by ID-LC–MS/MS
por: Beasley-Green, Ashley, et al.
Publicado: (2023)

A rapid, simple and sensitive LC-MS/MS method for lenvatinib quantification in human plasma for therapeutic drug monitoring
por: Zanchetta, Martina, et al.
Publicado: (2021)

Rapid and accurate method for quantifying busulfan in plasma samples by isocratic liquid chromatography-tandem mass spectrometry (LC-MS/MS)
por: Villena-Ortiz, Yolanda, et al.
Publicado: (2022)

Trace Level Quantification of 4-Methyl-1-nitrosopiperazin in Rifampicin Capsules by LC-MS/MS
por: Tao, Xiaosha, et al.
Publicado: (2022)

New method for quantification of gasotransmitter hydrogen sulfide in biological matrices by LC-MS/MS
por: Tan, Bo, et al.
Publicado: (2017)

Global profiling of gut microbiota-associated lipid metabolites in antibiotic-treated mice by LC-MS/MS-based analyses
por: Okahashi, Nobuyuki, et al.
Publicado: (2021)

Quantification of cGMP in human plasma by isotope dilution LC-MS/MS
por: Martens-Lobenhoffer, Jens, et al.
Publicado: (2009)

LC-MS/MS Quantification of Tramadol and Gabapentin Utilizing Solid Phase Extraction
por: Nagaraju, Pappula, et al.
Publicado: (2018)

Fast and Simple LC-MS/MS Method for Rifampicin Quantification in Human Plasma
por: Temova Rakuša, Žane, et al.
Publicado: (2019)

Modern Approaches in the Identification and Quantification of Immunogenic Peptides in Cereals by LC-MS/MS
por: Alves, Thais O., et al.
Publicado: (2019)

LC-MS/MS for Ultra-Sensitive Quantification of Multiple Estrogens in the Blood and Brain
por: Jalabert, Cecilia, et al.
Publicado: (2021)

Redox Metabolism Measurement in Mammalian Cells and Tissues by LC-MS
por: Petrova, Boryana, et al.
Publicado: (2021)

Development, Validation, and Comparison of Two Mass Spectrometry Methods (LC-MS/HRMS and LC-MS/MS) for the Quantification of Rituximab in Human Plasma
por: Millet, Aurélien, et al.
Publicado: (2021)

Protocol for determining the regulation of lipid kinases and changes in phospholipids in vitro
por: Karabiyik, Cansu, et al.
Publicado: (2021)

A novel accurate LC-MS/MS method for quantitative determination of Z-lumirubin
por: Jašprová, Jana, et al.
Publicado: (2020)

LC-MS/MS Method for Rapid Quantification of Progesterone in Rabbit Plasma and Its Application in a Pharmacokinetic Study of the Transdermal Formulation
por: Tran, Cao-Son, et al.
Publicado: (2020)

A Rapid LC-MS/MS-PRM Assay for Serologic Quantification of Sialylated O-HPX Glycoforms in Patients with Liver Fibrosis
por: Panigrahi, Aswini, et al.
Publicado: (2022)

Analysis of polar primary metabolites in biological samples using targeted metabolomics and LC-MS
por: Turtoi, Evgenia, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_44ne6vc8pkee9fmsd5p5hf417j