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Real-Time fast PCR amplification using designated and conventional real time thermal cycler systems: COVID-19 perspective
The study aimed to shorten multiplex RT-PCR run time for detection of SARS CoV-2 N1 and N2 sequences and human RNase P (RP) sequence as internal mRNA control using conventional and designated real time thermal cycler systems. Optimization of Fast PCR protocol using plasmid-based N1 and N2 positive c...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9584428/ https://www.ncbi.nlm.nih.gov/pubmed/36265002 http://dx.doi.org/10.1371/journal.pone.0276464 |