Cargando…

Real-Time fast PCR amplification using designated and conventional real time thermal cycler systems: COVID-19 perspective

The study aimed to shorten multiplex RT-PCR run time for detection of SARS CoV-2 N1 and N2 sequences and human RNase P (RP) sequence as internal mRNA control using conventional and designated real time thermal cycler systems. Optimization of Fast PCR protocol using plasmid-based N1 and N2 positive c...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hossain, Md. Walid, Hossain, Mohabbat, Arafath, Khalid, Ety, Subarna Sayed, Shetu, Md. Mahade Hasan, Kabir, Mazbahul, Noor, Farjana Akther, Mannoor, Kaiissar
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2022
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9584428/ https://www.ncbi.nlm.nih.gov/pubmed/36265002 http://dx.doi.org/10.1371/journal.pone.0276464

Ejemplares similares

Development and performance evaluation of the first in-house multiplex rRT-PCR assay in Bangladesh for highly sensitive detection of SARS-CoV-2
por: Noor, Farjana Akther, et al.
Publicado: (2021)

Nationwide carrier detection and molecular characterization of β-thalassemia and hemoglobin E variants in Bangladeshi population
por: Noor, Farjana Akther, et al.
Publicado: (2020)

Detection of Ochratoxin a Using Molecular Beacons and Real-Time PCR Thermal Cycler
por: Sanzani, Simona Marianna, et al.
Publicado: (2015)

A real-time PCR assay for the detection and quantitation of Campylobacter jejuni using SYBR Green I and the LightCycler.
por: Yang, Chengbo, et al.
Publicado: (2004)

Recent Advances in Understanding the Role of Genomic and Epigenomic Factors in Noncommunicable Diseases
por: Shekhar, Hossain U., et al.
Publicado: (2019)

Frequency of Hepatitis B, C and HIV Infections among Transfusion-Dependent Beta Thalassemia Patients in Dhaka
por: Bhuyan, Golam Sarower, et al.
Publicado: (2021)

Identification and validation of reference genes for real-time quantitative RT-PCR analysis in jute
por: Hossain, Md. Sabbir, et al.
Publicado: (2019)

Monkeypox virus detection in rodents using real-time 3′-minor groove binder TaqMan® assays on the Roche LightCycler
por: Kulesh, David A, et al.
Publicado: (2004)

Human immunodeficiency virus type 1 (HIV-1) proviral DNA load in purified CD4+ cells by LightCycler(® )Real-time PCR
por: Kabamba-Mukadi, Benoît, et al.
Publicado: (2005)

Real-Time Multirate Multiband Amplification for Hearing Aids
por: SOKOLOVA, ALICE, et al.
Publicado: (2022)

Rapid identification of Bordetella pertussis pertactin gene variants using LightCycler real-time polymerase chain reaction combined with melting curve analysis and gel electrophoresis.
por: Mäkinen, J, et al.
Publicado: (2001)

Evaluation of Type-Specific Real-Time PCR Assays Using the LightCycler and J.B.A.I.D.S. for Detection of Adenoviruses in Species HAdV-C
por: Jones, Morris S., et al.
Publicado: (2011)

Ultrasensitive Nonenzymatic Real-Time Hydrogen Peroxide Monitoring Using Gold Nanoparticle-Decorated Titanium Dioxide Nanotube Electrodes
por: Kader, Md. Ashraful, et al.
Publicado: (2023)

Whole genome amplification and real-time PCR in forensic casework
por: Giardina, Emiliano, et al.
Publicado: (2009)

Investigation of the impact of nonsynonymous mutations on thyroid peroxidase dimer
por: Begum, Mst. Noorjahan, et al.
Publicado: (2023)

COVID-19 and Bangladesh: Situation report, comparative analysis, and case study
por: Saifuzzaman, Mohd, et al.
Publicado: (2021)

Detection of Cassava Component in Sweet Potato Noodles by Real-Time Loop-mediated Isothermal Amplification (Real-time LAMP) Method
por: Wang, Deguo, et al.
Publicado: (2019)

Deep Learning-Based IoT System for Remote Monitoring and Early Detection of Health Issues in Real-Time
por: Islam, Md. Reazul, et al.
Publicado: (2023)

Evaluation of Senna tora (L.) Roxb. leaves as source of bioactive molecules with antioxidant, anti-inflammatory and antibacterial potential
por: Rahman, Md. Mashiar, et al.
Publicado: (2023)

Real-Time Loop-Mediated Isothermal Amplification (RealAmp) for the Species-Specific Identification of Plasmodium vivax
por: Patel, Jaymin C., et al.
Publicado: (2013)

Quantification of Trace-Level DNA by Real-Time Whole Genome Amplification
por: Kang, Min-Jung, et al.
Publicado: (2011)

Real-Time Fluorescence Loop Mediated Isothermal Amplification for the Diagnosis of Malaria
por: Lucchi, Naomi W., et al.
Publicado: (2010)

Novel Bioluminescent Quantitative Detection of Nucleic Acid Amplification in Real-Time
por: Gandelman, Olga A., et al.
Publicado: (2010)

REAL Real-Time!
por: Boeckman, F, et al.
Publicado: (2000)

Genetic variation for induced and basal resistance against leaf pathogen Pseudomonas syringae pv. tomato DC3000 among Arabidopsis thaliana accessions
por: Hossain, Md Motaher, et al.
Publicado: (2015)

Evaluation of Sensitivity and Specificity of Three Commercial Real-Time Quantitative Polymerase Chain Reaction Kits for Detecting SARS-CoV-2 in Bangladesh
por: Mim, Farzana, et al.
Publicado: (2021)

Trends and advances in food analysis by real-time polymerase chain reaction
por: Salihah, Nur Thaqifah, et al.
Publicado: (2016)

Impact of COVID-19 on mental health: A quantitative analysis of anxiety and depression based on regular life and internet use
por: Rahman, Md. Mosfikur, et al.
Publicado: (2021)

Molecular Zipper: a fluorescent probe for real-time isothermal DNA amplification
por: Yi, Jizu, et al.
Publicado: (2006)

An inter-platform repeatability study investigating real-time amplification of plasmid DNA
por: Donald, Carol E, et al.
Publicado: (2005)

Real-Time Fluorescence Loop Mediated Isothermal Amplification for the Detection of Acinetobacter baumannii
por: Wang, Qinqin, et al.
Publicado: (2013)

Real-Time Duplex Applications of Loop-Mediated AMPlification (LAMP) by Assimilating Probes
por: Kubota, Ryo, et al.
Publicado: (2015)

Instrument for Real-Time Digital Nucleic Acid Amplification on Custom Microfluidic Devices
por: Selck, David A., et al.
Publicado: (2016)

Real-time loop-mediated isothermal amplification for rapid detection of Enterocytozoon hepatopenaei
por: Cai, Shao-Xin, et al.
Publicado: (2018)

Rapid detection of Mucorales based on recombinase polymerase amplification and real-time PCR
por: Xu, Rui, et al.
Publicado: (2023)

Investigation of respiratory disease outbreaks of poultry in Bangladesh using two real-time PCR-based simultaneous detection assays
por: Parvin, Rokshana, et al.
Publicado: (2022)

Real-time PCR in detection and quantitation of Leishmania donovani for the diagnosis of Visceral Leishmaniasis patients and the monitoring of their response to treatment
por: Hossain, Faria, et al.
Publicado: (2017)

Quantifying genome DNA during whole-genome amplification via quantitative real-time multiple displacement amplification
por: Tu, Jing, et al.
Publicado: (2021)

Scrutinizing Deleterious Nonsynonymous SNPs and Their Effect on Human POLD1 Gene
por: Bappy, Md. Nazmul Islam, et al.
Publicado: (2022)

Real time.
por: Axbey, Susan
Publicado: (1997)

Cannot write session to /tmp/vufind_sessions/sess_4glms1lajhacdusbvkiahmf4pk