Development of a versatile high-throughput mutagenesis assay with multiplexed short-read NGS using DNA-barcoded supF shuttle vector library amplified in E. coli

A forward mutagenesis assay using the supF gene has been widely employed for the last several decades in studies addressing mutation frequencies and mutation spectra associated with various intrinsic and environmental mutagens. In this study, by using a supF shuttle vector and non-SOS-induced Escher...

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Detalles Bibliográficos
Autores principales: Kawai, Hidehiko, Iwata, Ren, Ebi, Shungo, Sugihara, Ryusei, Masuda, Shogo, Fujiwara, Chiho, Kimura, Shingo, Kamiya, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Cell Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9584611/
https://www.ncbi.nlm.nih.gov/pubmed/36214452
http://dx.doi.org/10.7554/eLife.83780

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9584611/
https://www.ncbi.nlm.nih.gov/pubmed/36214452
http://dx.doi.org/10.7554/eLife.83780

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