Comprehensive analysis and accurate quantification of unintended large gene modifications induced by CRISPR-Cas9 gene editing

Most genome editing analyses to date are based on quantifying small insertions and deletions. Here, we show that CRISPR-Cas9 genome editing can induce large gene modifications, such as deletions, insertions, and complex local rearrangements in different primary cells and cell lines. We analyzed larg...

Descripción completa

Detalles Bibliográficos
Autores principales: Park, So Hyun, Cao, Mingming, Pan, Yidan, Davis, Timothy H., Saxena, Lavanya, Deshmukh, Harshavardhan, Fu, Yilei, Treangen, Todd, Sheehan, Vivien A., Bao, Gang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2022
Materias:
Biomedicine and Life Sciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9586483/
https://www.ncbi.nlm.nih.gov/pubmed/36269834
http://dx.doi.org/10.1126/sciadv.abo7676

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9586483/
https://www.ncbi.nlm.nih.gov/pubmed/36269834
http://dx.doi.org/10.1126/sciadv.abo7676

Ejemplares similares