Comprehensive analysis and accurate quantification of unintended large gene modifications induced by CRISPR-Cas9 gene editing

Most genome editing analyses to date are based on quantifying small insertions and deletions. Here, we show that CRISPR-Cas9 genome editing can induce large gene modifications, such as deletions, insertions, and complex local rearrangements in different primary cells and cell lines. We analyzed larg...

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Detalles Bibliográficos
Autores principales: Park, So Hyun, Cao, Mingming, Pan, Yidan, Davis, Timothy H., Saxena, Lavanya, Deshmukh, Harshavardhan, Fu, Yilei, Treangen, Todd, Sheehan, Vivien A., Bao, Gang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2022
Materias:
Biomedicine and Life Sciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9586483/
https://www.ncbi.nlm.nih.gov/pubmed/36269834
http://dx.doi.org/10.1126/sciadv.abo7676

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