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Extended depth-of-field light-sheet microscopy improves imaging of large volumes at high numerical aperture

Light-sheet microscopes must compromise among field of view, optical sectioning, resolution, and detection efficiency. High-numerical-aperture (NA) detection objective lenses provide higher resolution, but their narrow depth of field inefficiently captures the fluorescence signal generated throughou...

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Detalles Bibliográficos
Autores principales: Keomanee-Dizon, Kevin, Jones, Matt, Luu, Peter, Fraser, Scott E., Truong, Thai V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9586705/
https://www.ncbi.nlm.nih.gov/pubmed/36276589
http://dx.doi.org/10.1063/5.0101426