Development of an in vivo cleavable donor plasmid for targeted transgene integration by CRISPR-Cas9 and CRISPR-Cas12a

The CRISPR-Cas system is widely used for genome editing of cultured cells and organisms. The discovery of a new single RNA-guided endonuclease, CRISPR-Cas12a, in addition to the conventional CRISPR-Cas9 has broadened the number of editable target sites on the genome. Here, we developed an in vivo cl...

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Detalles Bibliográficos
Autores principales: Ishibashi, Riki, Maki, Ritsuko, Kitano, Satsuki, Miyachi, Hitoshi, Toyoshima, Fumiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9588054/
https://www.ncbi.nlm.nih.gov/pubmed/36272994
http://dx.doi.org/10.1038/s41598-022-22639-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9588054/
https://www.ncbi.nlm.nih.gov/pubmed/36272994
http://dx.doi.org/10.1038/s41598-022-22639-6

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