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Establishment of in vivo proximity labeling with biotin using TurboID in the filamentous fungus Sordaria macrospora

Proximity-dependent biotin identification (BioID) has emerged as a powerful methodology to identify proteins co-localizing with a given bait protein in vivo. The approach has been established in animal cells, plants and yeast but not yet in filamentous fungi. BioID relies on promiscuous biotin ligas...

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Detalles Bibliográficos
Autores principales: Hollstein, Lucas S., Schmitt, Kerstin, Valerius, Oliver, Stahlhut, Gertrud, Pöggeler, Stefanie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9588061/
https://www.ncbi.nlm.nih.gov/pubmed/36272986
http://dx.doi.org/10.1038/s41598-022-22545-x