Engineered helicase replaces thermocycler in DNA amplification while retaining desired PCR characteristics

Polymerase Chain Reaction (PCR) is an essential method in molecular diagnostics and life sciences. PCR requires thermal cycling for heating the DNA for strand separation and cooling it for replication. The process uses a specialized hardware and exposes biomolecules to temperatures above 95 °C. Here...

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Detalles Bibliográficos
Autores principales: Gavrilov, Momčilo, Yang, Joshua Y. C., Zou, Roger S., Ma, Wen, Lee, Chun-Ying, Mohapatra, Sonisilpa, Kang, Jimin, Liao, Ting-Wei, Myong, Sua, Ha, Taekjip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9588791/
https://www.ncbi.nlm.nih.gov/pubmed/36274095
http://dx.doi.org/10.1038/s41467-022-34076-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9588791/
https://www.ncbi.nlm.nih.gov/pubmed/36274095
http://dx.doi.org/10.1038/s41467-022-34076-0

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