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Engineered helicase replaces thermocycler in DNA amplification while retaining desired PCR characteristics
Polymerase Chain Reaction (PCR) is an essential method in molecular diagnostics and life sciences. PCR requires thermal cycling for heating the DNA for strand separation and cooling it for replication. The process uses a specialized hardware and exposes biomolecules to temperatures above 95 °C. Here...
Autores principales: | Gavrilov, Momčilo, Yang, Joshua Y. C., Zou, Roger S., Ma, Wen, Lee, Chun-Ying, Mohapatra, Sonisilpa, Kang, Jimin, Liao, Ting-Wei, Myong, Sua, Ha, Taekjip |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9588791/ https://www.ncbi.nlm.nih.gov/pubmed/36274095 http://dx.doi.org/10.1038/s41467-022-34076-0 |
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