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High-throughput mass cytometry staining for deep phenotyping of human natural killer cells

This protocol details the step-by-step procedure for in-depth immune phenotyping of peripheral blood natural killer (NK) cells from clinical samples by mass cytometry. The protocol consists of three main steps: PBMC incubation with a mix of metal-conjugated antibodies for extracellular phenotyping f...

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Detalles Bibliográficos
Autores principales: Ben Amara, Amira, Rouviere, Marie-Sarah, Fattori, Stéphane, Wlosik, Julia, Gregori, Emilie, Boucherit, Nicolas, Bernard, Pierre-Louis, Nunès, Jacques A., Vey, Norbert, Luche, Herve, Gorvel, Laurent, Olive, Daniel, Chretien, Anne-Sophie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9589031/
https://www.ncbi.nlm.nih.gov/pubmed/36269638
http://dx.doi.org/10.1016/j.xpro.2022.101768