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High-throughput mass cytometry staining for deep phenotyping of human natural killer cells
This protocol details the step-by-step procedure for in-depth immune phenotyping of peripheral blood natural killer (NK) cells from clinical samples by mass cytometry. The protocol consists of three main steps: PBMC incubation with a mix of metal-conjugated antibodies for extracellular phenotyping f...
| Autores principales: | , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9589031/ https://www.ncbi.nlm.nih.gov/pubmed/36269638 http://dx.doi.org/10.1016/j.xpro.2022.101768 |
| _version_ | 1784814208213319680 |
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| author | Ben Amara, Amira Rouviere, Marie-Sarah Fattori, Stéphane Wlosik, Julia Gregori, Emilie Boucherit, Nicolas Bernard, Pierre-Louis Nunès, Jacques A. Vey, Norbert Luche, Herve Gorvel, Laurent Olive, Daniel Chretien, Anne-Sophie |
| author_facet | Ben Amara, Amira Rouviere, Marie-Sarah Fattori, Stéphane Wlosik, Julia Gregori, Emilie Boucherit, Nicolas Bernard, Pierre-Louis Nunès, Jacques A. Vey, Norbert Luche, Herve Gorvel, Laurent Olive, Daniel Chretien, Anne-Sophie |
| author_sort | Ben Amara, Amira |
| collection | PubMed |
| description | This protocol details the step-by-step procedure for in-depth immune phenotyping of peripheral blood natural killer (NK) cells from clinical samples by mass cytometry. The protocol consists of three main steps: PBMC incubation with a mix of metal-conjugated antibodies for extracellular phenotyping followed by fixation, permeabilization and incubation with a mix of metal-conjugated antibodies for staining of intracellular proteins, and sample acquisition on a mass cytometer. High-dimensional analysis enables the visualization of NK cell subsets and their phenotypical characteristics. For complete details on the use and execution of this protocol, please refer to Chretien et al. (2021). |
| format | Online Article Text |
| id | pubmed-9589031 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2022 |
| publisher | Elsevier |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-95890312022-10-25 High-throughput mass cytometry staining for deep phenotyping of human natural killer cells Ben Amara, Amira Rouviere, Marie-Sarah Fattori, Stéphane Wlosik, Julia Gregori, Emilie Boucherit, Nicolas Bernard, Pierre-Louis Nunès, Jacques A. Vey, Norbert Luche, Herve Gorvel, Laurent Olive, Daniel Chretien, Anne-Sophie STAR Protoc Protocol This protocol details the step-by-step procedure for in-depth immune phenotyping of peripheral blood natural killer (NK) cells from clinical samples by mass cytometry. The protocol consists of three main steps: PBMC incubation with a mix of metal-conjugated antibodies for extracellular phenotyping followed by fixation, permeabilization and incubation with a mix of metal-conjugated antibodies for staining of intracellular proteins, and sample acquisition on a mass cytometer. High-dimensional analysis enables the visualization of NK cell subsets and their phenotypical characteristics. For complete details on the use and execution of this protocol, please refer to Chretien et al. (2021). Elsevier 2022-10-20 /pmc/articles/PMC9589031/ /pubmed/36269638 http://dx.doi.org/10.1016/j.xpro.2022.101768 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| spellingShingle | Protocol Ben Amara, Amira Rouviere, Marie-Sarah Fattori, Stéphane Wlosik, Julia Gregori, Emilie Boucherit, Nicolas Bernard, Pierre-Louis Nunès, Jacques A. Vey, Norbert Luche, Herve Gorvel, Laurent Olive, Daniel Chretien, Anne-Sophie High-throughput mass cytometry staining for deep phenotyping of human natural killer cells |
| title | High-throughput mass cytometry staining for deep phenotyping of human natural killer cells |
| title_full | High-throughput mass cytometry staining for deep phenotyping of human natural killer cells |
| title_fullStr | High-throughput mass cytometry staining for deep phenotyping of human natural killer cells |
| title_full_unstemmed | High-throughput mass cytometry staining for deep phenotyping of human natural killer cells |
| title_short | High-throughput mass cytometry staining for deep phenotyping of human natural killer cells |
| title_sort | high-throughput mass cytometry staining for deep phenotyping of human natural killer cells |
| topic | Protocol |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9589031/ https://www.ncbi.nlm.nih.gov/pubmed/36269638 http://dx.doi.org/10.1016/j.xpro.2022.101768 |
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